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AutoPure ADA 33
₹9,026.00
Overview
Buy
AutoPure ADA 33 Reagent
from Accurex (R1 1 X 22ml: R2 1 X 11ml). ADA catalyzes deamination of adenosine to inosine which is then converted to hypoxanthine by purine nucleoside phosphorylase (PNP).
Hypoxanthine is then converted to uric acid and hydrogen peroxide (H,0) by xanthine oxidase (XOD). The ADA Reagent kit should be stored at 2-8°C and is stable till the expiry. Accurex Biomedical is the best reagents manufacturing company in India.
50 in stock
About
AutoPure ADA can be used on various automated analyzers. General procedure is as described below.
ADA catalyzes deamination of adenosine to inosine which is then converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H,0) by xanthine oxidase (XOD).H,0, is further reacted with N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine (4 AAP) in the presence of peroxidase (POD) to generate quinone dye.
Calibration frequency Re-calibration is recommended as per the requirement of quality control procedures.
Principle
ADA catalyzes deamination of adenosine to inosine which is then Converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H,0,) by xanthine oxidase (XOD).H,0, is further reactedwith N – Ethyl-N-2-hydroxy-3-sulfopropyl!)-3-methylaniline (EHSPT) and 4-aminoantipyrine (4 AAP) in the presence of peroxidase (POD) to generate quinone dye.
Procedure
Reaction direction…………………………. Increasing
Wavelength …………….. ….. 546 nm.
Flow Cell temperature …………………… 37°C
Zero setting with …… Distilled water
Delay time.. . ..300 seconds
No. of readings…………4
Interval………………… 60 seconds
Sample volume. ………….. 10 ul
Reagent 1 (R1) volume…………. 360 ul
Reagent 2 (R2) volume…… 180 ul
Factor. ……….. 1743
Linearity … …………..200 U/L
Quality Control
Each batch of AutoPure ADA is assayed with multiple quality control sera prior to release.
To ensure adequate quality control,it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together. If the control values fall outside the specified limits each of the below criteria should be cross- checked and corrected:
-Proper instrument function -wavelength setting, light source and temperature control
-Cleanliness of probes and cuvettes.
-Bacterial contamination of wash water used by the instrument.
-Expiry date of the reagent kit
Other Details
- The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
- The reagent and standard are ready-to-use and are stable till expiry, when stored at 2- 8°C. DO NOT FREEZE THE REAGENT.
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