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Diluent Glucose STAT 2000
₹1,500.00
Overview
Buy Autozyme Diluent Glucose STAT 2000 Diagnostic Reagent (Pack Size: 10 X 200ml) from Accurex. Diluent Glucose STAT reagent is used for determination of true glucose based on enzymatic method using Glucose oxidase and peroxidase. estimates glucose in 1 minute, using an initial rate method & in 7 minutes by end point method. Intravenous sugar solution, also known as dextrose solution, is a mixture of dextrose (glucose) & water. Diluent Glucose 2000 is used to treat
low blood sugar
or water loss without electrolyte loss, water loss without electrolyte loss may occur in fever, hyperthyroidism, high blood calcium, or diabetes insipidus.
4 in stock
About
- AutoZyme STAT Glucose is a reagent set for determination of True Glucose based on enzymatic methods using Glucose oxidase and Peroxidase.
- AutoZyme STAT Glucose estimates glucose in just one minute using an initial rate method and in 7 minutes by end-point method.
- AutoZyme STAT Glucose pack sizes 10 x 100 ml and 4 x 500 ml are provided with 2 standards of 100 mg% and 300 mg%.
- AutoZyme STAT Glucose is Linear up to 700 mg% glucose for kinetic assay procedure and 500 mg% using end-point procedure.
- AutoZyme STAT Glucose is a High Stability Reagent.
- AutoZyme STAT Glucose can be used on any Spectrophotometer, Discrete semi automated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
- AutoZyme STAT Glucose has one step reconstitution. It involves the mixing of ENZYME and DILUENT.
- Sodium Fluoride (as an anticoagulant up to 10 mg/ml blood) does not elect glucose assay.
- The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Hemoglobin is negligible.
- Very small volume of serum or plasma is required for assay.
- The Shelf-life of AutoZyme STAT Glucose is 18 months.
Principle
Glucose oxidase (GOD) converts Glucose to gluconic acid. Hydrogen
peroxide formed in this reaction, in presence of Peroxidase (POD),
oxidatively couples with 4-aminoantipyrine / phenol to produce red
quinoneimine dye. This dye has absorbance maximum at 505 nm.
(500-550 nm.). The intensity of the color complex is directly proportional to the Glucose in the specimen.
Procedure
Reaction type…..2 Point-Kinetic
Reaction direction………………Upward
Wavelength…………..505 nm. (500- 550 nm.)
Flow cell temperature ……37°C
Zero setting with……Reagent Blank
Delay time…………………………..20 secs
No. of readings…………………..2
Interval……………………………………..40 secs.
Blank absorbance limit……………..< 0.300 Abs.
Sample volume…………0.01 ml (10)
Reagent volume……….1.0 ml
Factor……………..100 / (A Abs. of std.)
Linearity…………………..700 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of the test include proper instrument function, temperature control cleanliness of glassware and accuracy of pipetting.
Other Details
Stability of the reconstituted working reagent is printed on the bottle label.
(DO NOT FREEZE). The working solution should be stored in the dark bottle (diluent bottle or the working solution bottle) provided. This is critical because the reagent is light sensitive (auto oxidation of chromogen system by light and air), it should therefore be kept away from direct light.
1 review for Diluent Glucose STAT 2000
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Dipen Patel –
Reagents quality really appreciable, only prices should be competitive