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BUN 250
₹3,480.00
Overview
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Infinite Liquid BUN/UREA 250 Diagnostic Reagent
(R1 4 X 50ml: R2 2 X 25ml) from Accurex. Liquid BUN/UREA reagent kit is used for determination of blood urea nitrogen (BUN) concentration in serum or plasma based on Enzymatic UV- Kinetic initial rate method.
Liquid BUN is a ready to use – two liquid reagent system, linear upto 250 mg/dl & estimates urea nitrogen concentration in just 1 minute. The rate of decrease in absorbance at 340 nm is directly proportional to BUN Concentration in the specimen.
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About
- Infinite Liquid BUN (BUN 250 – Liquid) is a reagent set for determination of blood urea nitrogen (BUN) concentration in serum or plasma based on Enzymatic UV- Kinetic initial rate method.
- Infinite Liquid BUN is a ready-to-use, two liquid reagent system.
- Infinite Liquid BUN estimates urea nitrogen concentration in just 1 minute.
- Infinite Liquid BUN is linear upto 250 mg/dl.
- Infinite Liquid BUN can be used on any Spectrophotometer, Discrete Semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request
- Infinite Liquid BUN is stable till expiry at 2 -8°C.
Method – GLDH-Kinetic
Pack Size : R1: 4 x 50 ml R2:2x25ml
Principle
Urea is hydrolysed to ammonia and carbon dioxide by urease. Ammonia produced reacts with o-ketoglutarate to form glutamate in presence of glutamate dehydrogenase. NADHİs oxidized to NAD in this reaction, which is measured as a decrease in absorbance at 340 nm. The rate of decrease in absorbance at 340 nm. is directly proportional to BUN Concentration in the specimen.
Procedure
Reaction type …………………………. UV – Kinetic
Reaction direction …………………………. Decreasing
Wavelength ………………………. 340 nm.
Flow Cell temperature ………………………. 25°C/30°C
Zero setting with ………………………. Distilled
water Delay time ………………………. 30 seconds
No. of readings ………………………. 2
Interval ………………………. 30 seconds
Blank absorbance limit ………………………. 21.000 Abs.
Sample volume ………………………. 0.01 ml (10 ul)
Working solution volume (4 R, : 1 R.) – 1.0 ml (1000 ul)
Factor – 20 = (A Abs. of Standard)
Linearity – 250 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and abnormal commercial reference control serum.
It should be realized that the use of quality cOntrol material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature Control, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagent kit should be stored at 2- 89C and is stable till the expiry date indicated on the label. R and R, reagents are stable till expiry at 2- 8°C. The working solution (4 R 1 R.) is stable for 30 days at 2-8°C.
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