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BUN 200
₹2,784.00
Overview
Buy Autozyme
BUN/UREA (GLDH) 200 Diagnostic Reagent
(Pack Size : 20 x 5 ml) from Accurex. BUN/UREA reagent is used for determination of urea nitrogen based on enzymatic UV-Kinetic initial rate method using urease and glutamate dehydrogenase. Autozyme BUN/UREA is a two reagent system using one procedure, can be determined in just 5 minutes.
Blood Urea Nitrogen
is a single reagent system using one step procedure.
BUN/UREA concentration in the specimen is determined by comparing the sample reaction rate to that obtained, with a BUN standard. Normal BUN ranges around 6 to 24 mg/dL (2.1 to 8.5 mmol/L)
5 in stock
About
- Autozyme BUN is a reagent set for determination of urea nitrogen based on enzymatic UV-Kinetic initial rate method using urease and glutamate dehydrogenase
- Autozyme BUN is a single reagent system using one step procedure
- Autozyme BUN is linear up to 250 mg% of urea nitrogen
- Autozyme BUN has no influence of bilirubin, hemoglobin and amino acids in serum
Pack Size : 20 x 5 ml
Principle
Urea is hydrolyzed to ammonia and carbon dioxide by urease. Ammonia produced reacts with @-ketoglutarate to form glutamate in presence of glutamate dehydrogenase. NADH is oxidized to NAD* in this reaction, which Is measured as a decrease in absorbance at 340 nm. The rate of decrease in absorbance at 340 nm. is directly proportional to BUN concentration in the specimen. The BUN concentration in the specimen is determined by comparing the sample reaction rafe to that obtained with a BUN standard
Procedure
Reaction type ….. UV – Kinetic
Reaction direction ………………….. Down
Wavelength …………….. 340 nm.
Flow Cell temperature ………… 25°C/30°C
Zero setting with ……………. Distilled water
Delay time……………… 30 seconds
No. of readings ………….2
Interval ………….. 30 seconds
Blank absorbance limit … ………. 2 1.000 Abs.
Sample volume ……. 0.01 ml (10 ul)
Reagent volume ……………………………………………… 1.0 ml
Factor …… ..20 (4 Abs. of std.)
Linearity………… 250 mg%
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect include proper instrument function, temperature, the performance of this test control cleanliness of glassware and accuracy of pipetting.
Other Details
The reagent kit should be stored at 2- 8 C and is stable till the expiry date indicated on the label. The working solution is stable for 45 days at 2- 8°C.
1 review for BUN 200
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Krishnakant Kulkarni –
I’m the Accurex customer last 4 year, using Reagent and Semi Automated machine, good quality