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Total Protein 100
₹536.00
Overview
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Autozyme Total Protein 100 Diagnostic Reagent
(Pack Size : 2 x 50 ml) from Accurex. Total Protein reagent is used for determination of total protein based on Biuret method. Total protein is a single reagent system, ready to use & can be determined in five minutes.
Total protein normal range is 6.0 to 8.3 grams per deciliter (g/dL) or 60 to 83 g/L. total protein high in blood increases the concentration of protein in the bloodstream. High total protein level could indicate dehydration or certain type of cancer, such as multiple myeloma that causes protein to accumulate abnormally.
5 in stock
About
- Autozyme Total Protein is a reagent set for determination of total protein based on Biuret method
- Total Protein can be determined in five minutes
- Autozyme Total Protein is linear up to 18 mg%
- Autozyme Phosphorus is a single reagent system, ready to use
- The shelf life of Autozyme Total Protein is 18 months
- Autozyme Total Protein does not require serum blank for lipemic samples
Pack Size : 2 x 50 ml
Principle
Proteins react with cupric ions under alkaline pH to produce a color complex. This color complex absorbs light at 546 nm.(530-570 nm.). The intensity of the color is directly proportional to the protein concentration in the specimen.
Procedure
Reaction type ……….. End-Point
Reaction time ………………………………………….. 5 mins. at 37°C
Wavelength ………… 546 nm.(530-570 nm.)
Zero setting with …….. ………. Reagent Blank
Sample volume ………. .. 0.01 ml (10 ul) 0
Reagent volume ……………………. 1.0 ml 0
Standard concentration ……. ……… 6 gm%
Linearity .. …….. 18 gm%
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
Vaļue is stable in serum for 24 hours at 2-8°C and 30 days
when stored at-20°C.
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