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Alkaline Phosphatase 300
₹3,535.00
Overview
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Autozyme Alkaline Phosphatase 300 Diagnostic Reagent
from Accurex (Pack Size : 30 x 10 ml). Alkaline Phosphatase reagent is used for determination of Alkaline Phosphate activity based on kinetic method using p-nitrophenyl phosphate (p-NPP).
Alkaline Phosphatase is a single reagent system using one step procedure & Alkaline Phosphatase activity can be determined in just 2.5 minutes & is is a Highly stable reagent. Alkaline Phosphatase cleaves p-nitrophenyl phosphate p- NPP in the presence of magnesium ions to form p nitrophenol & phosphate.
About
- About
Method – p-NPP-DEA Buffer
– Kinetic reaction with 60 seconds lag phase
– Easy reconstitution : Tablet + Diluent
– High liquid stability : 21 days - Autozyme Alkaline Phosphatase is a reagent set for determination of Alkaline Phosphate activity based on kinetic method using p-nitrophenyl phosphate (p-NPP)
- Autozyme Alkaline Phosphatase is a single reagent system using one step procedure
- Autozyme Alkaline Phosphatase has one step reconstitution. It involves dissolving substrate tablet in diluent
- Autozyme Alkaline Phosphatase is a Highly stable reagent
- Autozyme Alkaline Phosphatase activity can be determined in just 2.5 minutes
Principle
Alkaline Phosphatase cleaves p-nitrophenyl phosphate p- NPP)
in the presence of magnesium ions to form p nitrophenol and
phosphate.
The intensity of the yellow color formed is directly proportional to the Application alkaline phosphatase activity in the specimen and measured kinetically.
Procedure
Reaction type……………Kinetic
Reaction direction…………… Up
Wavelength……………405 nm
Flow cell temperature……………37°C(±0.2°C)
Zero setting with……………Working solution
Delay time……………60 seconds
No. of readings……………4
Interval……………30 seconds
Blank absorbance limit……………< 0.700 Abs.
Sample volume……………0.02 ml (20 µl)
Reagent volume…………..1.0 ml
Factor……………2720
Linearity…………….700 IUl/l
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
- Two liquid reagent systems.
- Stable till the expiry date indicated on the label when stored in
2-8°C.
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