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Uric Acid 50
₹1,100.00
Overview
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Infinite Liquid Uric Acid 50 Diagnostic Reagent
(Pack Size: 2 X 25 ml) from Accurex. Liquid Uric Acid reagent kit is used for determination of Uric Acid based on enzymatic methods using Uricase and Peroxidase.
Influence of Glucose, Bilirubin, Ascorbate, Allopurinol, Urea, Protein, EDTA, Fluoride, Citrate, Oxalate & Hemoglobin is negligible. Uric Acid 50 Reagent is linear upto 25 mg%. Uric acid treatment can be either with medications or by intake of certain food items.
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About
- Infinite Liquid Uric Acid 125 is a reagent set for determination of Uric Acid based on enzymatic methods using Uricase and Peroxidase.
- Infinite Liquid Uric Acid 125 is a ready-to-use reagent.
- Infinite Uric Acid 125 assay can be performed in 5 minutes at 37°C or 10 minutes at R.T. (25 -30°C).
- Infinite Liquid Uric Acid 125 is linear upto 25 mg%.
- Infinite Liquid Uric Acid 125 can be used on any Colorimeter, Spectrophotometer, Discrete semi automated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
- The influence of Glucose, Bilirubin, Ascorbate, Allopurinol, Urea, Protein, EDTA, Fluoride, Citrate, Oxalate and Hemoglobin is negligible.
Method – Uricase
Pack Size : 2 x 25 ml
Principle
Uricase converts uric acid into allantoin and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidatively couples with phenolic chromogens to form a red coloured compound, which has maximum absorbance at 510 nm.(500-530 nm). The concentration of the red colored Compounds are proportional to the amount of uric acid in specimens.
Procedure
Reaction type ………….. End-Point
Reaction time ……5 mins. at 37°C/10 mins. at R.T. (25-30°C)
Wavelength ……………………….510 nm. (500 – 530 nm.)
Zero setting with ………………………………………. Reagent Blank
Blank absorbance limit …………….. < 0.300 Abs.
Sample volume …………… 0.025 ml (25 ul)
Reagent volume …………………… 1.0 ml
Standard concentration ………………… 6 mg% D
Linearity ………………… 25 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The kit should be stored at 2-8°C and is stable till the expiry
date indicated on the label.
The reagent and standard are ready-to-use and are stable till expiry, when stored at 2-89C. DO NOT FREEZE THE REAGENT.
The reagent should be stored only in the amber bottle provided to protect It is from direct light. Before using, swirl the reagent gently. DO NOT SHAKE VIGOROUSLY.
Over time, the reagent may develop a light pink color. This is expected and does not affect the reagent performance. Discard the reagent it the absorbance of the same exceeds 0.300 O.D. against distilled water At 510 nm. Contamination of the reagent should be strictly avoided. Should the reagent develop turbidity and discard the reagent.
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nidhi shah –
Accurex is a good Company. I am using it’s products since last 7 years.