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Glucose Eco-Pak 500
₹716.00
Overview
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Autozyme Glucose Eco-Pak 500 Diagnostic Reagent
(Pack Size : 5 x 100 ml) from Accurex. Glucose Eco-Pak 500 reagent is used for determination of true glucose based on enzymatic method using Glucose oxidase and peroxidase.
Glucose Eco-Pak 500 estimates glucose in 1 minute, using an initial rate method & in 7 minutes by end point method. Glucose is linear up to 700mg% glucose for kinetic assay procedure and 500mg% using end point procedure & has one step reconstitution. It involves mixing Enzyme and Diluent.
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About
- Eco-Pak is a reagent set for determination of true glucose based on enzymatic method using Glucose oxidase and peroxidase
- Eco-pak Glucose can be determined in just 15 minutes at 37 degree C or 30 minutes at RT
- Eco-pak Glucose is linear up to 450 mg% and has high stability
- Eco-Pak Glucose has one step reconstitution. It involves mixing Enzyme and Diluent
Pack Size : 5 x 100 ml
Principle
Glucose oxidase (GOD) converts glucose to gluconic acid. Hydrogen peroxide formed in this reaction, in presence of peroxidase (POD), oxidatively couples with 4-aminoantipyrine and phenol to produce red quinoneimine dye. This dye has absorbance maximum at 505 nm. (500 – 550 nm.). The intensity of the color complex is directly proportional to the concentration of glucose in the specimen.
Procedure
Reaction type ………… End-Point
Reaction time …. 15 mins. at 37°C/30 mins. at R.T. (25 -30°C)
Wavelength ……………….. 505 nm.(500-550 nm.)
Zero setting with ……….. ……….. Working Solution
Sample volume …………………………….0.01 ml (10 ul)
Reagent volume ………..1.0 ml
Standard concentration …… ……… 100 mg%
Linearity ……….. …………… 450 mg/dl
Quality Control
To ensure adequate quantity control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use to quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The working solution is stable for 60 days when stored at 2 -8°C (DO NOT FREEZE). The working solution should be stored in the dark bottle (working solution container) provided. This is critical because the reagent is light sensitive (auto oxidation of chromogen system by light and air), it should therefore be kept away from direct light.
5 reviews for Glucose Eco-Pak 500
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