About
PRINCIPLE AND REFERENCE VALUES
The Blood Lipid Test Strip (Dry Chemistry) use a timed-endpoint method to measure the Total Cholesterol
(TC), High Density Lipoprotein Cholesterol (HDL) and Triglycerides (TG) concentrations in whole blood,
serum or plasma. The concentration of Low Density Lipoprotein Cholesterol (LDL) is calculated by the
values of TC, TG and HDL. The system monitors the change in absorbance at 620 nm at a fixed-time
interval. The change in absorbance is directly proportional to the concentration of lipid in the specimen.
TC: The free cholesterol is oxidized to cholesten-3-one and hydrogen peroxide by cholesterol oxidase.
Peroxidase catalyzes the reaction of hydrogen peroxide with 4-aminoantipyrine and phenol to produce a
colored quinoneimine product.
HDL: The dextran slulphate/Mg2+ on the test strip precipitates the chylomicrons, VLDL and LDL, leaving
HDL in the specimen. The cholesterol concentration of this HDL is then determined enzymatically, the
same as TC.
TG: Triglycerides in the specimen are hydrolyzed to glycerol and free fatty acids by the action of lipase.
A sequence of three coupled enzymatic steps using glycerol kinase (GK), glycerophosphate oxidase
(GPO), and peroxidase (POD) causes the oxidative coupling of 4-aminoantipyrine to form a blue dye.
LDL; When the concentration of TG in the specimen is equal to or lower than 400mg/dL, LDL
concentration can be calculated by the meter with the following equation:
LDL=TC-HDL-TG/2.2(mmol/L); LDL=TC-HDL-TG/5(mg/dL)
Specification
INTENDED USE
The Blood Lipid Test Strip (Dry Chemistry) work with the Blood Lipid Analysis Meter or Dry
Biochemical Analysis Meter to measure the lipid concentration in whole blood, plasma and serum.
For professional use or self-testing use.
The 3-1 Lipid Panel is used to measure the concentrations of Total Cholesterol (TC), High Density
Lipoprotein Cholesterol (HDL) and Triglycerides (TG). It is also used to calculate LDL, TC/HDL.
Lipid measurements are used in the diagnosis and treatment of atherosclerotic coronary artery
disease and in the diagnosis of metabolic disorders involving lipids and lipoproteins.
Direction for Use
DIRECTIONS FOR USE
Allow the test strip, specimen, and/or controls to reach operating temperature (10-35℃) prior to
testing.
Refer to the Blood Lipid Monitoring System or Dry Biochemical Monitoring System User’s Manual
for detailed instructions.
1. Insert the code chip into the meter and code the meter correctly. Refer to coding the meter section
in the User’s Manual for details. Compare the code number on the code chip with the code number
printed on the test strip canister or foil pouch and ensure the two numbers are identical to avoid
inaccurate results.
2. Check that the specimen type is same as the specimen type tested. If not, set the correct specimen
type. Refer to the User’s Manual for details. Remove the test strip from the test strip canister or
foil pouch.
3. Wait for the meter to display Insert Strip. Insert the test strip completely into the test strip channel
in the same direction as the arrows printed on the test strip.
4. Prepare the specimen to be tested. For venous whole blood/plasma/serum specimens: mix the
specimen for about 15minutes. For capillary blood specimen: wipe away the first drop of blood.
Collect 35μL of the second or third drop of capillary blood specimen using a capillary transfer tube
or pipette. Refer to the User’s Manual for details. Hold the tube slightly downward and touch the
tip of the capillary transfer tube to the blood drop.
Draw the specimen and stop drawing when the specimen comes to the fill line.
5. While the meter is flashing the blood drop symbol, apply 35μL specimen to the Specimen
Application Area of the test strip using a pipette or capillary transfer tube. Align the tip of the
pipette or capillary transfer tube with the Specimen Application Area to apply the blood. 4 dashed
lines will appear on the meter to show the test is in progress.
6. Read the results on the screen in 2 minutes. Refer to the User’s Manual for detail test procedures.
Other Info
STORAGE AND STABILITY
Store as packaged in the sealed pouch or canister, either at room temperature or refrigerated (2-30℃).
Keep out of direct sunlight. Test strips are stable through the expiration date printed on the test strip
canister or foil pouch.
Remove only enough test strips for immediate use. Replace the cap on the strips canister immediately
and tightly.
Do not freeze. Do not use it beyond the expiration date
Chetan Patil –
I am using this last 3 years easy friendly product gives accurate result