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Creatinine 200
₹1,365.00
Overview
Buy Autozyme
Creatinine 200 Diagnostic Reagent
(Pack Size : 2 x 500 ml) from Accurex. Creatinine 200 reagent is used for determination of creatinine based on initial rate method using Alkaline Picrate. creatinine levels: typical range for serum creatinine is: For adult men, 0.74 to 1.35 mg/dL (65.4 to 119.3 micromoles/L), for adult women, 0.59 to 1.04 mg/dL (52.2 to 91.9 micromoles/L).
Creatinine can be determined in 180 seconds & this working solution is stable for 30 days at 2-8°C. Autozyme Creatinine 200 is a single reagent system, using one step procedure.
About
- Autozyme Creatinine is a reagent set for determination of creatinine based on initial rate method using Alkaline Picrate
- Autozyme Creatinine is a single reagent system, using one step procedure
- Autozyme Creatinine has one step reconstitution. It involves mixing of Picrate and Diluent reagent
- Creatinine can be determined in 180 seconds
- Autozyme Creatinine is linear up to 15 mg/dL
Pack Size : 2 x 500 ml
Principle
Creatinine in an alkaline medium reacts with pirates to produce an orange color.
This color absorbs light at 492 nm. (490 – 510 nm.. The rate of increase in absorbance is directly proportional to the concentration of creatinine in specimens.
Procedure
Reaction type……………. Reaction direction Initial rate Up
Wavelength ………………… 492 nm.(490 – 510 nm.)
Flow Cell…………………….. temperature 30°C / 37°C
Zero setting with ………………….. Distilled water
Delay time ………………. 30 seconds
No. of readings ………………… 2
Interval ……………………… 60 seconds
Sample volume ……………………….0.05 ml (50 111)
Reagent volume ……………….. 1.0 ml
Standard concentration …………………. 2 mg%
Factor …………………. 2 A Abs. of standard
Linearity ……………….. 30 mg/d1
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. t should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature Control, cleanliness of glassware, and accuracy of pipetting.
Other Details
The reagents are stable till the expiry date stated on the bottle label, when stored at R.T. (25-30°C).
The working solution is stable for 30 days at 2-8°C.
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