Principle
Calcium forms a purple color complex with cresol phthalein complexone in alkaline medium. This complex absorbs light at 575 (570 – 580) nm. The intensity of the color is directly proportional to the calcium concentration in the specimen.
Procedure
Reaction type………………. End-Point
Reaction time………………….. 5 mins. at R.T. (25-30°C)
Wavelength…………………… 575 nm.(570 – 58 aim.)
Zero setting with ……………………… Reagent Blank
Sample volume……………………. 0.02 ml (20 11 1 )
Reagent volume…………………………….. 1.0 ml
Standard concentration ……………….10 mg%
Linearity…………………………… 15 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagents are stable till the expiry date stated on the bottle label, when stored at 2-8°C.
The working solution is stable for 7 days at 2-8°C.
Ashish Tambe –
Quality Products & Quality Service