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About
- AutoPure HDL-C is a reagent kit for direct quantitative determination of HDL-C in human serum and plasma on automated clinical chemistry analyzers.
- AutoPure HDL-Cis ready-to-use, two liquid reagent system.
- With AutoPure HDL-C, the assay is linear upto 150 mg/dl
Principle
AutoPure HDL-Cholesterol assay is a homogenous method for directly measuring HDL-C levels in the serum or plasma without the need for any off-line pretreatmentor centrifugation steps.
Procedure
AutoPure HDL-C can be used on various automated analyzers
Quality Control
Each batch of AutoPure HDL-C is assayed with multiple quality control sera prior to release. To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together. If the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:
• Proper instrument function – wavelength setting, light source and temperature control.
• Cleanliness of probes and cuvettes.
• Bacterial contamination of wash water used by the instrument.
• Expiry date of the reagent kit.
Other Details
- The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
- The reagent and standard are ready-to-use and are stable till expiry, when stored at 2- 8°C. DO NOT FREEZE THE REAGENT.
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