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HDL – CHOL 100 (Precipitant)
₹1,018.00
Overview
Buy Autozyme HDL – CHOL 100 (Precipitant) (Pack Size : 2 x 50 ml) from Accurex. HDL – CHOL 100 (Precipitant) reagent is used for determination of Inorganic phosphorus based on UV-Kinetic method using Ammonium molybdate. Phosphotungstate/Mg2+ precipitates chylomicrons, LDL and VLDL fractions. High-Density
Lipoprotein
(HDL) fraction remains unaffected in supernatant.
5 in stock
About
- AutoZyme HDL-Cholesterol Precipitating Reagent is for use in conjunction with AutoZyme Cholesterol Reagent – for enzymatic determination of HDL-Cholesterol in serum or plasma.
- Compared to the conventional Ultracentrifugation method, the precipitation method is simple and time saving; particularly when combined with single-step enzymatic AutoZyme Cholesterol reagent.
Principle
Phosphotungstate/Mg2+ precipitates chylomicrons, LDL and VLDL
fractions. High Density Lipoprotein (HDL) fraction remains unaffected in supernatant. Cholesterol content of HDL fraction is assayed using AutoZyme Cholesterol.
Procedure
Reaction type ………………………… End-Point
Reaction time…………..10 mins. at 37°0/30 mins. at R.T. (25-30°C)
Wavelength …………….. 510 nm. (505-53O nm)
Zero setting with ………………………. Reagent Blank
Blank absorbance limit ………………. < 0.100 Abs.
Sample volume………0.05 ml (50 ul)
Reagent volume …… 1.0 ml
Standard Concentration ………… 50 mg%
Linearity ………. 400 mg%
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together, Factors which might effect the performance of this test include proper instrument function, temperature
control, cleanliness of glassware and accuracy of pipetting.
Other Details
HDL-Cholesterol vaļue is stable in serum for 24 hours at 2-8°C and 30 days when stored at-20°C.
1 review for HDL – CHOL 100 (Precipitant)
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Sahin –
I am using your biochemistry reagents. Last one year, good products, good quality.