About
- AutoPure Gamma – GT is a reagent kit for direct quantitative determination of gamma – glutamyltransferase (GGT) in human serum and plasma on automated clinical chemistry analyzers.
- AutoPure Gamma-GT is a ready-to-use, two liquid reagent system.
- With AutoPure Gamma – GT, the assay is linear upto 1200 IU/ (20.04 ukat/l).
Principle
Fully automated systems automatically calculate the Gamma-GT activity of each sample. Results in ukat/l = Results in IU/Ix0.0167
Gamma – glutamyltransferase (GGT) transfers Y – glutamyl group of L-Y-glutamyl-3-carboxy-4-nitroanilide to glycylglycine. L-Y-glutamyl-3-carboxy-4-nitroanilide + glycylglycine.
GGT Y-glutamyl-glycyl glycine + 5-amino-2-nitrobenzoate
With AutoPure Gamma-GT, the assay is linear upto 1200 10/ (20.04 ukat/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCl or distilled/deionized water (e.g. 1+4). Multiply the result by the appropriate dilution factor (e.g.5).
Application Sheet
Refer to the application sheet for details. For additional system applications, contact our local Accurex representative.
Calibration
There is no significant interference in samples containing upto 30 mg/dl of bilirubin, 90 mg/dl of haemoglobin and 10 mg/dl of ascorbic acid.
The amount of 5-amino-2-nitrobenzoate liberated is proportional to the GGT activity in the specimen and is measured kinetically. Reagent Storage, Stability & Handling
For calibration, it is recommended to use C.f.a.s* or any other suitable calibrator material.
Procedure
Autopure Glucose (Hexokinase) can be used on various
automated analyzers.
Quality Control
On-Board Reagent Stability R1:21 days at 20-8°C after opening. R2:21 days at 20-8°C after opening. Protect the reagent from light and contamination.
Other Details
The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
The reagent and standard are ready-to-use and are stable til expiry, when stored at 2- 8°C. DO NOT FREEZE THE REAGENT.
1 review for AutoPure Gamma GT 60
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Kevin –
I like product and super quality, normal cost