Principle
Bilirubin reacts with diazotized sulfanilic acid in acidic medium to
produce azobilirubin. DMSO catalyzes the formation of azobilirubin
from free bilirubin. DMSO
Bilirubin + Sulphanilic acid + Sodium NitriteAzobilirubin
Direct bilirubin, being water soluble directly reacts in acid c med um to produce Azobilirubin.
Bilirubin Sulphanilic acid + Sodium Nitrite Azobilirubin
The intensity of the viciet co our formed is directly proportional to the Concentration of bilirubin in the specimen and is measured
photometrically.
Procedure
Reaction type……………………. End-Point E
Reaction time ………………….5 mins. at R.T. (25-30°C)
Wavelength……………………………546 nm.(530 – 550 nm.)
Zero setting with…………………….Serum Blank
Sample volume ……………………… 0.05 ml (50 III)
Reagent volume…………………..1.02 ml
Factor …………………………………20.2
Linearity …………………………20 mg%
Quality Control
Each batch of AutoPure Bilirubin is assayed with at least six quality control sera – Precinom Precipath*, Bio Rad |” Biorad It* Accutrol Normal and Accutrol Abnormal prior to release
To ensure adequate quality control, it is recommended that the laboratory should Use a normal and abnormal commercial reference control serum. It should Be realized that the use of quality control material checks both the reagent and
instrument functions together.
H the control values fall outside the specified limits, each of the below criteria should be cross checked and corrected:
-Proper instrument function wavelength setting, light sourCe and temperature
-Cleanliness of probes anc cuvettes.
-Bacterial contamination of wash water used by the instrument
-Expiry date of the reagent kt.
-Brand naTIE of Roche Diagnostics, CermaryBrand name a Biorad. USA,subiect to
availability of control serum.
Ramesh singh –
Best quality reagent
Ranjit –
Having worked extensively with the Bilirubin Direct 164 Reagent in our clinical laboratory, I can confidently attest to its outstanding performance and reliability in the determination of Total and Direct Bilirubin levels.
Nitin –
We have found that the Bilirubin Direct 164 Reagent consistently delivers precise results across a wide range of sample types, making it a reliable choice for routine clinical testing.