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AutoPure Bilirubin Total 164
₹3,355.00
Overview
Buy
AutoPure Bilirubin 164 Reagent
from Accurex (R1 8 X 20ml: R2 1 X 4ml). Bilirubin 164 Reagent is used for the determination of Total and Direct Bilirubin based on Jendrassik & Grof method, using Diazotized Sulfanilic Acid with DMSO as an activator for Total Bilirubin.
Bilirubin is a two reagent system using one procedure, linear up to 20mg% & can be determined in just 5 minutes. Autozyme Bilirubin method is relatively free of interference from hemoglobin and other commonly occuring substances in serum/plasma.
5 in stock
About
- Method – Jendrassik & Grof
- Ready-to-use liquid stable reagents
- Simple test procedure : 1.0 ml + 0.02 ml + 0.05 ml
- Results via factor : 20.2 at 530 nm. – 550 nm.
Pack Size : 1 x 164 ml
Principle
Bilirubin Reacts with diazotized sulfanilic add in acidic medium to
Precipitate produce azobilirubin, DMSO catalyzes the formation of azobilirubin from free bilirubin.
The intensity of the violet color formed is directly proportional to the Concentration of bilirubin in the specimen and is measured
photometrically.
Procedure
Reaction type ………………Fixed point
Reaction direction…………… ………….. Increasing
Wavelength ……………..540 nm.
Flow Cell temperature ………………..37°C
Zero setting with ……………………………………. Distilled water
Delay time ………. ……………..5 seconds
No. of readings ……………………………2
Interval ………………………………………. ………….120 seconds
Sample volume ………… ………………7 ul
Working solution volume (4R1 + 1R2) …………………1.0 ml
Linearity ……… ……………………150 mg/L
Quality Control
Each batch of AutoPure Bilirubin is assayed with at least six quality control sera – Precinom Precipath*, Bio Rad |” Biorad It* Accutrol Normal and Accutrol Abnormal prior to release
To ensure adequate quality control, it is recommended that the laboratory should USe a normal and abnormal commercial reference control serum. t should Be realized that the use of quality control material checks both the reagent and instrument functions together.
H the control values fall outside the specified limits, each of the below criteria should be cross checked and corrected:
-Proper instrument function wavelength setting, light source and temperature
-Cleanliness of probes anc cuvettes.
-Bacterial contamination of wash water used by the instrument
-Expiry date of the reagent kt.
-Brand naTIE of Roche Diagnostics, CermaryBrand name a Biorad. USA, subject to availability of control serum.
Other Details
- The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
- The reagent and standard are ready-to-use and are stable till expiry, when stored at 2- 8°C. DO NOT FREEZE THE REAGENT.
2 reviews for AutoPure Bilirubin Total 164
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Manish Upadhyay –
Ready-to-use reagent and are stable till expiry
Pranav –
We have found the Accurex Bilirubin 164 Reagent to be remarkably user-friendly, with clear and concise instructions that facilitate seamless integration into our laboratory workflow.