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AutoPure Amylase 40
₹8,104.00
Overview
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AutoPure Amylase 40 Reagent
from Accurex (2 X 20ml). Alkaline Phosphatase 100 Reagent is used for direct quantitative determination of a-Amylase in human security & plagina on automated clinical chemistry analyzers.
Amylase is a ready-to-use single liquid reagent system & assay is linear upto 2000 IU(33.4 phat). The Reagent is Stable till expiry date indicated on the label when stored at 2-8°C.
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About
- AutoPure Amylase is reagent kit for direct quantitative determination of Amylase in human security and plagina on automated clinical chemistry analyzers.
- AutoPure Amylase is a ready-to-use, single liquid reagent system
- With AutoPure Amylase the assay is linear upto 2000 IU (33.4 phat)
Principle
Amylase involves use of a chromogenic substrate Gal-G,-d-CNP 2- chloro-4-nitrophenyl linked with galactosyl maltoside). The Girec action of d-Amylase with this substrate results n ne release of more than 90% of 2-chloro-4-nitrophenol.
The intensity of the 2- chloro 4- nitrophenol formed is directly proportional to the -Amylase activity in the specimen and is Measured photometrically
Procedure
Reaction type ………………Fixed point
Reaction direction…………… ………….. Increasing
Wavelength ……………..540 nm.
Flow Cell temperature ………………..37°C
Zero setting with ……………………………………. Distilled water
Delay time ………. ……………..5 seconds
No. of readings ……………………………2
Interval ………………………………………. ………….120 seconds
Sample volume ………… ………………7 ul
Working solution volume (4R1 + 1R2) …………………1.0 ml
Linearity ……… ……………………150 mg/L
Quality Control
To ensure adequate quality control, it is recommended that each laboratory should use a normal and an abnormal commercial reference control material. It should be realized that the use of quality control material checks both reagent and instrument functions together.
Factors which might affect the performance of this test include instrument function, temperature control, cleanliness of glasswares and accuracy of pipetting.
Other Details
AutoPure a-Amylase is ready-to-use, single liquid reagent system.
Stable till date indicated on the label when stored at 2-8°C On-Board Reagent Stability 30 days at 2-8°C after opening Protect the reagent from light and Gorntanalon.
Do not freeze the reagent. Discard The reagent if the absorbance of the same exceeds 0.500OD. against distilled water at415 nm.
2 reviews for AutoPure Amylase 40
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Rajesh Shinde –
Good ready-to-use single liquid reagent
Nayan –
In terms of cost-effectiveness, the Alkaline Phosphatase 100 Reagent falls within a reasonable price range compared to similar products on the market.