About
- Fully automated systems automatically calculate the albumin concentration of each sample. Results in g/l = Results in g/dlx 10
- AutoPure Albumin is reagent kit for direct quantitative determination of albumin in human serum and plasma on automated clinical chemistry analyzers.
- AutoPure Albumin is a ready-to-use, single liquid reagent system.
- With AutoPure Albumin, the assay is linear upto 6 g/dl (60g/l).
- With AutoPure Albumin, the assay is linear upto 6 g/dl (60 g/l).
Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCl or distilled/deionized water (e.g 1 + 1). Multiply the result by the appropriate dilution factor (e.g. 2).
Principle
Serum albumin binds with the indicator bromocresol green (BCG) under acidic conditions to form a green coloured complex.
The intensity of the green colour formed is directly proportional to the concentration of albumin in the specimen and is measured photometrically.
Procedure
AutoPure Albumin can be used on various automated analyzers.
Quality Control
Each batch of AutoPure Albumin is assayed with at least six quality control sera — Precinorm**, Precipath**, Biorad l***, Biorad II***, Sero Low**** and Sero High**** prior to release.
To ensure adequate quality control, it is recommended that the laboratory should use normal and an abnormal commercial reference control serum.
It should be realized that the use of quality control material checks both reagent and instrument functions together. The values of these controls should fall within the specified limits. If control values fall outside specified limits, each of the below criteria should be cross-checked and corrected :
• Proper instrument function – wavelength setting, light source and
temperature control
• Cleanliness of probes & cuvettes
• Bacterial contamination of wash water used by the instrument
• Expiry date of the reagent kit **brand name of Roche Diagnostics, Germany; ***brand name of Biorad, USA ****brand name of Sero, Norway; subject to availability of control serum
Other Details
AutoPure Albumin is a ready-to-use, single liquid reagent system.
Stable till the expiry date indicated on the label, when stored at 15 – 25C.
Amit Sarkar –
This system reagent gives accurate result
Nilesh –
The Albumin 200 Reagent has been a staple in our lab for determining albumin levels in human serum and plasma, and it has consistently delivered reliable results.
Priyanka –
In terms of performance, the Albumin 200 Reagent consistently produces precise and reproducible results.