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ADA 60 – Infinite
₹20,423.00
Overview
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Infinite ADA 60 Diagnostic Reagent
(R1 1 X 20ml: R2 1 X 10 ml) from Accurex. Infinite ADA reagent is used for direct quantitative determination of Adenosine deaminase (ADA) activity in human serum, plasma and other body fluids.
The Reagent is a ready-to-use two liquid reagent system & can be performed in 11 minutes at 37° C. ADA is also know as Adenosine Deaminase which eliminates a molecule called deoxyadenosine, which is generated when DNA is broken down.
50 in stock
About
- Infinite ADA is a reagent set for direct quantitative determination of Adenosine deaminase (ADA) activity in human serum, plasma and other body fluids.
- Infinite ADA is a ready-to-use, two liquid reagent system.
- With Infinite ADA, the assay is linear upto 200 U/L.
- Infinite ADA assay can be performed in 11 minutes at 37° C.
Principle
ADA catalyzes deamination of adenosine to inosine which is then converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is further reacted with N-Ethyl-N-(2-hydroxy-3- sulfopropyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine (4 AAP) in the presence of peroxidase (POD) to generate quinone dye.
Procedure
• Reaction type…………………………………….Kinetic
• Reaction direction……………………………Increasing
• Wavelength………………………………………546 nm
• Flow Cell temperature……………………………..37° C
• Zero setting with……………………….Distilled water
• Delay time…………………………………300 seconds
• No. of readings………………………………………….4
• Interval………………………………………60 seconds
• Sample volume……………………………………..10 μl
• R 1 volume…………………………………………..360 μl
• R 2 volume…………………………………………180 μl
• Factor…………………………………………………1743
• Linearity……………………………………………200 U/L
Quality Control
To ensure adequate quality control, it is recommended that
each laboratory should use a normal and an abnormal
commercial reference control material. It should be realized
that the use of quality control material checks both reagent
and instrument functions together. Factors which might affect
the performance of this test include instrument function,
temperature control, cleanliness of glasswares and accuracy
of pipetting.
Other Details
The reagents R1 & R2 are ready-to-use.
The reagent kit should be stored at 2° – 8° C and is stable till
the expiry date indicated on the label.
Reagent R1 is light-sensitive & to be stored in dark.
Contamination of the reagents should be strictly avoided.
5 reviews for ADA 60 – Infinite
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Shubham –
Infinite ADA is also available in mono pack.
Shrutik Rajan –
Accurex is a quality based reagents manufacturing company .We are satisfied with your products
Amrut Singh –
reagent and rapid cards quality are superb.
Sagar Ram –
I am using Accurex produts 3 years I am satisfied with reagent good service
Sonali Gadkar –
Satisfied with reagent good service