Principle
Uricase converts uric acid into allantoin and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidatively couples with phenolic chromogens to form a red coloured compound, which has maximum absorbance at 510 nm.(500-530 nm). The concentration of the red colored Compounds are proportional to the amount of uric acid in specimens.
Procedure
Reaction type ………….. End-Point
Reaction time ……5 mins. at 37°C/10 mins. at R.T. (25-30°C)
Wavelength ……………………….510 nm. (500 – 530 nm.)
Zero setting with ………………………………………. Reagent Blank
Blank absorbance limit …………….. < 0.300 Abs.
Sample volume …………… 0.025 ml (25 ul)
Reagent volume …………………… 1.0 ml
Standard concentration ………………… 6 mg% D
Linearity ………………… 25 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The kit should be stored at 2-8°C and is stable till the expiry
date indicated on the label.
The reagent and standard are ready-to-use and are stable till expiry, when stored at 2-8°C. DO NOT FREEZE THE REAGENT.
The reagent should be stored only in the amber bottle provided to protect. It is from direct light. Before using, swirl the reagent gently. DO NOT SHAKEVIGOROUSLY.
Over time, the reagent may develop a light pink color. This is expected and does not affect the reagent performance. Discard the reagent it the absorbance of the same exceeds 0.300 O.D. against distilled water At 510 nm. Contamination of the reagent should be strictly avoided. Should the reagent develop turbidity and discard the reagent.
Seema Panchal –
We are using accurex Reagent And instruments last Five years. Products quality is very good.