Principle
Infinite Microalbumin Contains latex particles coated with specific anti human albumin antibodies which react with microalbumin in the sample resulting in agglutination. Agglutination causes change in absorbance, measured at 540 nm (530- 550 nm) & iS proportional to the concentration of microalbumin in the sample.
Procedure
Reaction type ………………Fixed point
Reaction direction…………… ………….. Increasing
Wavelength ……………..540 nm.
Flow Cell temperature ………………..37°C
Zero setting with ……………………………………. Distilled water
Delay time ………. ……………..5 seconds
No. of readings ……………………………2
Interval ………………………………………. ………….120 seconds
Sample volume ………… ………………7 ul
Working solution volume (4R1 + 1R2) …………………1.0 ml
Linearity ……… ……………………150 mg/L
Quality Control
To ensure adequate quality control, it is recommended that
each laboratory should use a normal and an abnormal
commercial reference control material. It should be realized
that the use of quality control material checks both reagent
and instrument functions together. Factors which might affect
the performance of this test include instrument function,
temperature control, cleanliness of glasswares and accuracy
of pipetting.
Other Details
The reagents R1 & R2 are ready-to-use.The reagent kit should be stored at 2- 8°C and is stable till the expiry date indicated on the label.
Salim Shaikh –
We are using accurex reagents and instruments. Very good product.