Principle
In the first step, HDL, VLOL & Chylomicrons are eliminated under specific conditions.
In the second step, special reagents break up LDL and make LDL Cholesterol available for measurement.
Procedure
Reaction type ……… End-Point (2 Step)
Reaction time ………………5 + 5 mins
Wavelength……………. 546 nm.
Flow Cell temperature……………….. 37°C
Zero setting with ……………………… Distilled water
Sample volume ……………. Q.01 ml (10 µt)
Reagent volume 0.750 ml + 0.250 ml
Linearity ………….. .. 450 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference SPECIMEN COLLECTION & PRESERVATION
Other Details
The reagent kit should be stored at 2 – 8°C and is stable till the expiry date indicated on the label. Reconstituted calibrator is stable for 3 days at 2′ – 8°C. Reconstitution stability of the calibrator may be extended upto 30 days by aliquoting and freezing the reconstituted calibrator preparation at less than -20°C.
Nancy K. –
quality of products impressive