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LDL 240 + HDL 240 – Autopure
₹24,378.00
Overview
Buy
AutoPure LDL 240 + HDL 240 Diagnostic Reagent
from Accurex.
HDL Cholestrol 120 (R1 2 X45ml: R2 2X 15ml) is used for direct quantitative determination of Low Density Lipoprotein Cholesterol (LDL-C) in human serum and plasma on automated clinical chemistry analyzers & LDL Cholestrol 250 (R1 4 X45ml: R2 4 X 15ml) is used for direct quantitative determination of HDL-C in human serum and plasma on automated clinical chemistry analyzers.
50 in stock
About
LDL:
– AutoPure LDL-C is a reagent kit for direct quantitative determination of Low Density Lipoprotein Cholesterol (LDL-C) in human serum and plasma on automated clinical chemistry analyzers.
– AutoPure LDL-Cis ready-to-use, two liquid reagent system.
– With AutoPure LDL-C, the assay is linear upto 450 mg/dl (11.637 mmol/l).
HDL:
– AutoPure HDL-C is a reagent kit for direct quantitative determination of HDL-C in human serum and plasma on automated clinical chemistry analyzers.
– AutoPure HDL-Cis ready-to-use, two liquid reagent system.
– With AutoPure HDL-C, the assay is linear up to 150 mg/dl
Principle
Specimen Collection & Preservation:
AutoPure LDL-Cholesterol assay is a homogenous method for directly measuring LDL-C levels in the serum or plasma without the need for any off-line pretreatment or centrifugation steps. In the first reaction, non LDL unesterified cholesterol is subject to an enzyme reaction and the peroxide generated is consumed by peroxidase in the presence of 4 – AAP to yield a colourless product. The second reagent consists of a detergent capable of solubilizing LDL specifically. Cholesterol esterase and chromogenic coupler react with this solubilized LDL-C to develop colour. The intensity of the colour formed is directly proportional to the concentration of the LDL-C in the specimen and is measured photometrically.
Collect sample using standard sampling tube. Serum, EDTA treated or sodium heparinized plasma are the recommended specimens. Patients are not required to fast prior to blood collection. Serum or plasma should not remain at 150-30°C for more than 14 hours. If assay is not completed within 14 hours, serum or plasma may be stored at 20- 8°C upto 1 week. If the specimens need to be stored for more than a week, they may be preserved at less than -70°C upto 3 months.
HDL:
AutoPure HDL-Cholesterol assay is a homogenous method for directly measuring HDL-C levels in the serum or plasma without the
need for any off-line pretreatment or centrifugation steps.
In the first reaction, non HDL unesterified cholesterol is subject to an enzyme reaction and the peroxide general ed is consumed by
peroxidase reaction with DSBmT to yield a colorless product. The
second reagent consists of a detergent capable of solubilizing HDL
Specifically. Cholesterol esterase ano chronic couper rcac Witn
this solubilized HDL G o develop coiournentersty cf the color omed is directly proportional lo he concession to the HDL specimen and is measured photometrically.
Procedure
LDL:
AutoPure LDL-C can be used on various automated analyzers.
HDL:
AutoPure HDL-C can be used on various automated analyzers.
Quality Control
LDL:
Each batch of AutoPure LDL-C is assayed with multiple quality control sera prior to release. To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together. If the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:
• Proper instrument function – wavelength setting, light source and temperature
control.
• Cleanliness of probes and cuvettes.
• Bacterial contamination of wash water used by the instrument.
• Expiry date of the reagent kit.
HDL:
Each batch of AutoPure HDL-C is assayed with multiple quality control sera prior to release.
To ensure adequate quality control, itis Recommended that the laboratory should bee domatand adenoma commercial reference control serum. It should be realized that the USe of quality control material checks both the reagent and Insinumet functions together.
1f the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:
– Proper instrument function wavelength setting, light source and temperatureControl
– Cleanliness of probes and cUvettes.
– Eacena arnitamnaton o waswa er usedarmenstument
– Expiry date of the reagent kit.
Other Details
LDL:
Refer to the application sheet for details. For additional system applications, contact our local Accurex representative.
With AutoPure LDL-C, the assay is linear up to 450 mg/dl (11.637 mmol/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCl or distilled/deionized water (e.g. 1 + 4). Multiply the result by the appropriate dilution factor (e.g.5)
AutoPure LDL-C is a ready-to-use, two liquid reagent system.
HDL:
AutoPure HD-Cis ready-to-USe, two liquid reagent system.
Stable till the expiry date indicated on the label when stored in
2-8°C.
1 review for LDL 240 + HDL 240 – Autopure
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Sarika Kahir –
We are using Accurex for last 8 yrs. Quality of the reagent is good.