About
- Infinite Liquid STAT Glucose (Glucose ‘STAT’ 1500 – Liquid) is a reagent set for determination of True Glucose, based on enzymatic method; using Glucose, oxidase and Peroxidase.
- Infinite Liquid STATGlucose is a ready-to-use reagent.
- Infinite Liquid STAT Glucose estimates glucose in 7 minutes at 37°C or 15 minutes at R.T. by end point method.
- Infinite Liquid STATGlucose is linear upto 500 mg%.
- Infinite Liquid STAT Glucose can be used on any Spectrophotometer, Discrete semi automated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
- Sodium Fluoride (as an anticoagulant upto 10 mg/ml blood) does not affect glucose assay.
- The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Hemoglobin is negligible.
Method – GOD-POD (End point & Kinectic)
Principle
Glucose oxidase (GOD) converts glucose to gluconic acid. Hydrogen
peroxide formed in this reaction, in presence of Peroxidase POD).
Oxidatively couples with 4-aminoantipyrine and phenol to produce red quinonimine dye. This dye has absorbance maximum at 505 nm. (500-550 nm),. The intensity of the color complex is directly proportional to the concentration of glucose in a specimen.
Procedure
Reaction type …………………….. End-Point
Reaction time ….. 7 mins. at 37°C / 15 mins. at R.T. (25-30°C)
Wavelength ……………….. 505 nm. (500 – 550 nm.)
Zero setting with .. ………… Reagent Blank
Sample volume …………. ………. 0.01 ml (10 ul)
Reagent volume …………. …………… 1.0 ml
Standard concentration ……. …………. 100 mg%
Linearity ……….. ………. 500 mg/di
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. t should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label. The reagent and standard are ready-to-use and are stable till expiry when stored at 2-8°C, DO NOT FREEZE THE REAGENT.
The reagent should be stored only in the amber bottle provided to protect it EE from direct light. This is critical because the reagent is light sensitive (auto Oxidation of chromogen systems by light and air). Before use swirl in the reagent gently. D0 NOT SHAKE VIGOROUSLY. Over time, the reagent may develop a light pink colour, this is expected and does not affect the reagent performance. Contamination of the reagent should be strictly avoided. Should the reagent develop turbidity and discard the reagent.
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