Principle
Glucose oxidase (GOD) converts glucose to gluconic acid. Hydrogen
peroxide formed in this reaction, in presence of Peroxidase (POD),
oxidatively couples with 4-aminoantipyrine and phenol to produce red quinonimine dye. This dye has absorbance maximum at 505 nm. (500-550 nm.), The intensity of the color complex is directly proportional to the concentration of glucOse in specimens.
Procedure
Reaction type …………………….. End-Point
Reaction time ….. 7 mins. at 37°C / 15 mins. at R.T. (25-30°C)
Wavelength ……………….. 505 nm. (500 – 550 nm.)
Zero setting with .. ………… Reagent Blank
Sample volume …………. ………. 0.01 ml (10 ul)
Reagent volume …………. …………… 1.0 ml
Standard concentration ……. …………. 100 mg%
Linearity ……….. ………. 500 mg/di
Quality Control
To ensure adequate quality control, it is recommended that
each laboratory should use a normal and an abnormal
commercial reference control material. It should be realized
that the use of quality control material checks both reagent
and instrument functions together. Factors which might affect
the performance of this test include instrument function,
temperature control, cleanliness of glasswares and accuracy
of pipetting.
Other Details
The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
The reagent and standard are ready-to-use and are stable till expiry when stored at 2-8°C, DO NOT FREEZE THE REAGENT.
The reagent should be stored only in the amber bottle provided to protect it from direct light. This is critical because the reagent is light sensitive (auto oxidation of chromogen systems by light and air). Before use swirl in the reagent gently.
DO NOT SHAKE VIGOROUSLY.
Over time, the reagent may develop a light pink colour, this is expected and does not affect the reagent performance.
Contamination of the reagent should be strictly avoided. Should the reagent develop turbidity discard the reagent
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