Principle
The serum sample is incubated with CK – MB reagent containing an antibody specific to CK-M subunit which completely inhibits the CK-M monomer. The activity of CK-B which is not inhibited by the antibody, is then measured by the following reaction sequence:
СК-В Creatine phosphate + ADP = creatine + ATP
Procedure
Reaction type …………… UV – Kinetic
Reaction direction …… .. Increasing
Wavelength …….. ……. 340 nm.
Flow Cell temperature. …
Zero setting with …. ………. Distilled water
Delay time ………………………………………………… 300 seconds
No. of readings … …………………………4
Interval ……………. …………….. 30 seconds
Sample volume ………………0.05 ml (50 ul)
Working solution volume (4R, : 1R) ……… 1.0 ml (1000 ul)
Factor ….. … 6752
Linearity 2000 IU/
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of the test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagent kit should be stored at 2 -8° C and is stable till the expiry date indicated on the label. R1 & R2 reagents are stable till expiry at 2 -8° C. The working solution (4 R1 + 1 R2) is stable for 10 days at 2 – 8° C.
Wellness Center –
Biomedical CK MB kit I’m using and I’m satisfied with this product. Just we need to take care of a storage