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Cholinesterase 18
₹1,236.00
Overview
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Infinite Liquid Cholinesterase 18ml Diagnostic Reagent
from Accurex (R1 1 X 15ml: R2 1 X 3ml). Liquid Cholesterol Reagent is used for determination of cholinesterase activity in human serum and plasma based on DGKC method using butyryl thiocholine. is a ready-to-use, two liquid reagent system.
Acetylcholinesterase
is known as AChE. AChase/ acetyl hydrolase is the primary cholinesterase in the body. Cholinesterase test can help determine your risk of poisoning.
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About
- Infinite Cholinesterase is a reagent kit for quantitative determination of cholinesterase activity in human serum and plasma based on DGKC method using butyryl thiocholine.
- Infinite Cholinesterase is a ready-to-use, two liquid reagent system.
- With Infinite Cholinesterase, the assay is linear upto 25000 U/L.
- Infinite Cholinesterase assay can be performed in 8 minutes at 37°C.
Principle
Cholinesterase catalyzes the hydrolysis of butyrylthiocholine, forming butyrate and thiocholine, which reduces the ferricyanide ions to ferrocyanide.
The decrease in absorbance is directly proportional to the activity of
cholinesterase in the specimen and is measured kinetically at 405 nm.
Procedure
Reaction type…………………. Kinetic
Reaction direction …………………. Decreasing
Wavelength…………………. 405 nm
Flow Cell temperature …………………. 37°C
Zero setting with …………………. Distilled water
Delay time …………………. 90 seconds
No. of readings …………………. 4
Interval …………………. 30 seconds
Sample volume…………………. 20 μl
R 1 volume…………………. 1000 μl
R 2 volume …………………. 200 μl
Factor …………………. 65800
Linearity …………………. 25000 U/L
Quality Control
To ensure adequate quality control, it is recommended that the
laboratory should use a normal and an abnormal commercial reference control material. It should be realized that the use of quality control material checks both reagent and instrument functions together.
Factors which might affect the performance of this test include
instrument function, temperature control, cleanliness of glassware’s
and accuracy of pipetting.
Other Details
The reagents R1 & R2 are ready-to-use.
The reagent kit should be stored at 2° – 8° C and is stable till the expiry date indicated on the label.
Keep the reagents away from direct light sources. Do not freeze the
reagent.
Contamination of reagent should be strictly avoided.
1 review for Cholinesterase 18
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Jyoti Joshi –
Experience with Accurex is excellent for reagents