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Cholesterol 250 – Infinite
₹3,749.00
Overview
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Infinite Liquid Cholesterol 250 Diagnostic Reagent
from Accurex (Pack Size: 5 X 50ml). Liquid Cholesterol Reagent is used for determination of total cholesterol based on enzymatic methods using Cholesterol esterase, Cholesterol oxidase & Peroxidase.
The Cholesterol Reagent is a ready-to-use reagent & can be determined in just 5 minutes at 37°C or 10 minutes at R.T. (25 -30°C). High cholesterol can lead to development of fatty deposits in your blood vessels.
50 in stock
About
- Infinite Liquid Cholesterol (Cholesterol 125 – Liquid) is a reagent set for determination of total cholesterol based on enzymatic methods using Cholesterol esterase, Cholesterol oxidase and Peroxidase.
- Infinite Liquid Cholesterol is a ready-to-use reagent.
- Infinite Cholesterol can be determined in just 5 minutes at 37°C or 10 minutes at R.T. (25 -30°C).
- Infinite Liquid Cholesterol is linear upto 1000 mg%.
- Infinite Liquid Cholesterol can be used on any Colorimeter Spectrophotometer, Discrete semi automated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
- The influence of Ascorbic acid, Bilirubin, Hemoglobin, Glucose, Sodium fluoride, Heparin, EDTA, Creatinine and Uric acid is negligible.
- Infinite Liquid Cholesterol reagent system can also be used for determining HDL Cholesterol. The HDL ppt reagent and standard can be ordered separately.
Method – CHOD-POD
Principle
Cholesterol esterase hydrolysis cholesterol esters into free cholesterol and fatty acids. In the second reaction cholesterol oxidase converts cholesterol to cholest-4-en-3-one and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidatively couples with 4- amino antipyrine and phenol to produce red quinonimine dye which has absorbance maximum at 510 nm. (500- 530). The intensity of the red color is proportional to the amount of total cholesterol in the specimen.
Procedure
Reaction type ……………….. End-Point (2 Step)
Reaction time ……………….. 5 + 5 mins.
Wavelength ……………….. 546 nm.
Flow Cell temperature……………….. 37°C
Zero setting with ……………….. Distilled water
Sample volume ……………….. Q.01 ml (10 μt)
Reagent volume ……………….. 0.750 ml + 0.250 ml
Linearity ……………….. 450 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that
each laboratory should use a normal and an abnormal
commercial reference control material. It should be realized
that the use of quality control material checks both reagent
and instrument functions together. Factors which might affect
the performance of this test include instrument function,
temperature control, cleanliness of glasswares and accuracy
of pipetting.
Other Details
The kit should be stored at 2- 8°C and is stable till the expiry date indicated on the label.
The reagent and standard are ready-to-use and are stable till expiry, when stored at 2- 8°C. DO NOT FREEZE THE REAGENT.
The reagent should be stored only in the amber bottle provided to protect it from direct light. Before using, swirl in the reagent gently. DO NOT SHAKE VIGOROUSLY.
Over time, the reagent may develop a light pink color. This is expected and does not affect the reagent performance. Discard the reagent if the absorbance of the same exceeds 0.300 O.D. against distilled water at 510 nm.
Contamination of the reagent should be strictly avoided. Should the reagent develop turbidity and discard the reagent.
1 review for Cholesterol 250 – Infinite
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Nakiyaa Parde –
Using Cholesterol 250 for 2years, Good products