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Cholesterol 125 – Infinite
₹2,282.00
Overview
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Infinite Liquid Cholesterol 125 Diagnostic Reagent
from Accurex (Pack Size: 5 X 25ml). Liquid Cholesterol Reagent is used for determination of total cholesterol based on enzymatic methods using Cholesterol esterase, Cholesterol oxidase & Peroxidase.
The Cholesterol Reagent is a ready-to-use reagent & can be determined in just 5 minutes at 37°C or 10 minutes at R.T. (25 -30°C). high cholesterol can lead to development of fatty deposits in your blood vessels.
50 in stock
About
- Infinite Liquid Cholesterol (Cholesterol 125 – Liquid) is a reagent set for determination of total cholesterol based on enzymatic methods using Cholesterol esterase, Cholesterol oxidase and Peroxidase.
- Infinite Liquid Cholesterol is a ready-to-use reagent.
- Infinite Cholesterol can be determined in ust 5 minutes at 37°C or 10 minutes at R.T. (25 -30°C).
- Infinite Liquid Cholesterol is linear up to 1000 mg%.
- Infinite Liquid Cholesterol can be used on any Colorimeter Spectrophotometer, Discrete semi automated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
- The influence of Ascorbic acid, Bilirubin, Hemoglobin, Glucose, Sodium fluoride, Heparin, EDTA, Creatinine and Uric acid is negligible.
- Infinite Liquid Cholesterol reagent system can also be used for determining HDL Cholesterol. The HDL ppt reagent and standard can be ordered separately.
Method – CHOD-POD
Pack Size : 5 x 25 ml
Principle
Cholesterol esterase hydrolysis cholesterol esters into free cholesterol and fatty acids. In the second reaction cholesterol oxidase converts cholesterol to cholest-4-en-3-one and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidatively couples with 4 amino antipyrine and phenol to produce red quinonimine dye which has absorbance maximum at 510 nm. (500 530), The intensity of the red color is proportional to the amount of total cholesterol in the specimen.
Procedure
Reaction type …………… End-Point (2 Step)
Reaction time …………….. 5 + 5 mins.
Wavelength ……………. 546 nm.
Flow Cell temperature…………… 37°C
Zero setting with ……………… Distilled water
Sample volume …………………. Q.01 ml (10 μt)
Reagent volume ……………. 0.750 ml + 0.250 ml
Linearity ………………………. 450 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The kit should be stored at 2- 8C and is stable till the expiry date indicated on the label.
The reagent and standard are ready-to-use and are stable till expiry when stored at 2- 8°C. DO NOT FREEZE THE REAGENT.
The reagent should be stored only in the amber bottle provided to protect it from direct light. Before using, swirl in the reagent gently. DO NOT SHAKEVIGOROUSLY.
Over time, the reagent may develop a light pink color. This is expected and does not affect the reagent performance. Discard the reagent if the absorbance of the same exceeds 0.300 O.D. against distilled water at 510 nm.
Contamination of the reagent should be strictly avoided. Should the reagent develop turbidity and discard the reagent.
5 reviews for Cholesterol 125 – Infinite
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Sameed Debnath –
Service &Product Quality good.
Sakti Madhur –
Using accurex Reagent last 8years. Good quality
Pramod Rane –
I have use all parameter biochemistry reagent .so this products quality and accuracy is very much
Mrunal Babu –
I am using accurex reagents.I am very much satisfied about the result and service
Shiva Shetty –
Good service and quality reagents…