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Calcium Arsenazo Monopack 50 Test
Overview
Buy Autozyme Calcium Arsenazo Monopack 50 test Diagnostic Reagent (Pack Size: 1x 50 ml) from Accurex. Calcium reagent is used for determination of total calcium concentration in serum based on the colorimetric method using Arsenazo III. In the Arsenazo III method the absorbance of the Ca-Arsenazo III complex is measured dichromatically at 660/700 nm. AutoZyme Calcium Arsenazo III is linear up to 15% mg.
About
Single Monovials Reagent
Kit storage at R.T.
End point assay: 2 minutes
Autozyme Calcium Arsenazo III is a reagent set for determination of total calcium concentration in serum based on the colorimetric method using Arsenazo III
Autozyme Calcium Arsenazo III is a ready to use reagent
Autozyme Calcium Arsenazo III estimates calcium in just 2 minutes at room temperature
Autozyme Calcium Arsenazo III is linear up to 15mg%
The influence of lipids, haemolysis and bilirubin is negligible. Pack Size : 1x 50 ml
Principle
Calcium with Arsenazo III at neutral pH yields a blue coloured complex. The intensity of the colour formed is directly proportional to calcium concentration.
Procedure
Reaction Type: End-Point
● Reaction Time: 2 mins at R.T. (25-30°C)
● Wavelength: 650 nm. (620-650 nm.)
● Zero setting with: Reagent Blank
● Blank absorbance limit: < 0.800 Abs.
● Sample volume: 0.02 ml (20 µl)
● Reagent volume: 1.0 ml
● Standard concentration: 10 mg %
● Linearity: 15 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control,
Other Details
Patients receiving EDTA treatment cannot be assayed for calcium correctly.
- If calcium value exceeds 15 mg% then dilute the specimen suitably with normal saline. In such a case the result obtained should be multiplied with the dilution factor to obtain the correct calcium value.
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