Blood Grouping Antisera

Direction for Use

TUBE TEST:

The reagent has been characterized by the recommended procedure. Before release, each of lot Anti A, B, and AB reagents is tested by the recommended procedures against a panel of antigen-positive red cells to ensure suitable reactivity.

The specificity of source monoclonal antibodies is shown using a panel of antigen-negative cells. Anti-B does not react with “Acquired-B” red cells. Monoclonal ABO reagents do not detect crypt antigens such as T, Tn, or Cad.

1. Place one drop of Anti-A, Anti-B, or Anti-AB on clean and dry slide.
2. Add one drop of whole blood or 40% RBC’s suspension prepared in the individual’s own serum
3. Mix well with an applicator stick; gently tilt the slide forward and backward at room temperature for a maximum of 2 minutes.

Tube Test:

1. Use an 8×50 mm small glass test tube for each specimen, take a tube and label it with the name or code number of the patient
2. Prepare 5% suspension of the RBCs to be tested in isotonic saline.
3. Add one drop of Anti-A, or Anti-B, or Anti-AB reagent and saline to the respective tubes
4. Add one drop of cell suspension to each tube and mix well.
5. Shake each tube thoroughly and centrifuge for 1 minute at 1000 RPM (125g)