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AutoPure Triglycerides 300
₹12,156.00
Overview
Buy AutoPure Triglycerides 300 (Pack Size: 6 X 50ml) from Accurex.
AutoPure Triglycerides 300 Reagent
is used for direct quantitative determination of triglycerides in human serum and plasma on automated clinical chemistry analyzers.
AutoPure Triglycerides 300 Reagent is a ready-to-use, single liquid reagent system & With Total Protein 80 the assay is linear upto 800 mg/dl (9.04 mmol/l).The AutoPure Triglycerides 300 kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
50 in stock
About
- AutoPure Triglycerides is a reagent kit for direct quantitative determination of triglycerides in human serum and plasma on automated clinical chemistry analyzers.
- AutoPure Triglycerides is ready-to-use, single liquid reagent system.
- With AutoPure Triglycerides, the assay is linear upto 800 mg/dl (9.04 mmol/l}.
- AutoPure Triglycerides contains lipid clearing factor (LCF) in its formulation. The LCF clears the turbidity caused by lipemic sample and thus avoids overestimation of triglycerides.
Principle
Lipoprotein lipase hydrolyses triglycerides to yield glycerol and fatty
acids. Glycerol kinase converts glycerol to glycerol-3-phosphate which is oxidized by glycerol phosphate oxidase to di- ydroxyacetone phosphate and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidatively couples with 4 aminoantipyrine and 4 – chlorophenol to produce red quinonimine dye. The intensity of the red colour formed is proportional to the concentration of triglycerides in the specimen and is measured photometrically.
Procedure
AutoPure Triglycerides can be used on various automated analyzers.
Quality Control
Each batch of AutoPure Triglycerides iis assayed with atleast six quality control sera Precinorm**, Precipath**, Biorad I***, Biorad II***, Accutrol Normal and Accutrol Abnormal prior to release-. To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together. If the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:
• Proper instrument function – wavelength setting, light source and temperature control.
• Cleanliness of probes and cuvettes.
• Bacterial contamination of wash water used by the instrument.
• Expiry date of the reagent kit. **Brand name of Roche Diagnostics, Germany; ***Brand name of Biorad, USA; subject to availability of control serum.
A comparison of the Total Protein determination using AutoPure Total Protein reagent and AutoZyme Total Protein gave the following correlation (g/dl): Linear Regression y=-0.201 + 1.030x r=0.996 Sy.x=0.10 No. of samples measured: 85
The sample concentrations measured were between 4 g/dl and 9 g/dl.
Other Details
AutoPure Triglycerides is a single ready-to-use reagent system. Stable till the expiry date indicated on the label, when stored at 2°-8°C.
5 reviews for AutoPure Triglycerides 300
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