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AutoPure Total Protein 80*
₹670.00
Overview
Buy AutoPure Total Protein 80 (Pack Size: 4 X 20ml) from Accurex.
AutoPure Total Protein 80 Reagent
is used for direct quantitative determination of total protein in human serum and plasma on automated clinical chemistry analyzers.
AutoPure Total Protein 80 Reagent is a ready-to-use, single liquid reagent system & With Total Protein 80 the assay is linear upto 18 g/dl (180g/). The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
50 in stock
About
– AutoPure Total Protein is a reagent kit for direct quantitative determination of total protein in human serum and plasma on automated clinical chemistry analyzers.
– AutoPure Total Protein is ready-to-use, single liquid reagent system.
– With AutoPure Total Protein the assay is linear upto 18 g/dl (180g/).
Principle
Fully automated system automatically calculates the total protein concentration of each sample. Results in g/1 = Results in g/dl x 10 With AutoPure Total Protein, the assay is linear up to 18 g/dl (180 g/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCl or distilled/deionized water (e.g. 1 + 3). Multiply the result by the appropriate dilution factor (e.g. 4).
Divalent copper ion reacts in alkaline solution with protein peptide bonds to form purple coloured biuret complex.
Procedure
AutoPure Total Protein can be used on various automated analyzers.
Quality Control
Each batch of AutoPure Total Protein is assayed with atleast six quality control sera Precinorm**, Precipath**, Biorad I***, Biorad II***, Accutrol Normal and Accutrol Abnormal prior to release-. To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together. If the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:
• Proper instrument function – wavelength setting, light source and temperature control.
• Cleanliness of probes and cuvettes.
• Bacterial contamination of wash water used by the instrument.
• Expiry date of the reagent kit. **Brand name of Roche Diagnostics, Germany; ***Brand name of Biorad, USA; subject to availability of control serum.
A comparison of the Total Protein determination using AutoPure Total Protein reagent and AutoZyme Total Protein gave the following co-relation (g/dl): Linear Regression y=-0.201 + 1.030x r=0.996 Sy.x=0.10 No. of samples measured: 85 The sample concentrations measured were between 4 g/dl and 9 g/dl.
Other Details
- The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
- The reagent and standard are ready-to-use and are stable till expiry, when stored at 2- 8°C. DO NOT FREEZE THE REAGENT.
2 reviews for AutoPure Total Protein 80*
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Jonatha Patidar –
Accurex reagent gives perfect result
Umesh –
This product gives accurate and precuse results. Value for Money !