AutoPure LDL Cholesterol 240

Principle

AutoPure LDL-Cholesterol assay is a homogenous method for directly measuring LDL-C levels in the serum or plasma without the need for any off-line pretreatment or centrifugation steps. In the first reaction, non LDL unesterified cholesterol is subject to an enzyme reaction and the peroxide generated is consumed by peroxidase in the presence of 4 – AAP to yield a colourless product. The second reagent consists of a detergent capable of solubilizing LDL specifically. Cholesterol esterase and chromogenic coupler react with this solubilized LDL-C to develop colour. The intensity of the colour formed is directly proportional to the concentration of the LDL-C in the specimen and is measured photometrically.
Collect sample using standard sampling tube. Serum, EDTA treated or sodium heparinized plasma are the recommended specimens. Patients are not required to fast prior to blood collection. Serum or plasma should not remain at 150-30°C for more than 14 hours. If assay is not completed within 14 hours, serum or plasma may be stored at 20- 8°C upto 1 week. If the specimens need to be stored for more than a week, they may be preserved at less than -70°C upto 3 months.