Principle
The amount of NADH formed is directly proportional to the
concentration of Glucose in the specimen and is measured
photometrically at 340 nm.
Procedure
Autopure Glucose (Hexokinase) can be used on various
automated analyzers.
Quality Control
Each batch of Autopure Glucose (Hexokinase) is assayed
with at least six control sera – Precinorm**, Precipath**,
Biorad I***, Biorad II***, Accutrol normal and Accutrol
Abnormal prior to release▲.
To ensure adequate quality control, it is recommended that
the laboratory should use a normal and an abnormal
commercial reference control material. It should be realized
that the use of quality control material checks both reagent
and instrument functions together.
If the control values fall outside the specified limits, each of
the below criteria should be cross-checked and corrected:
• Proper instrument function – wavelength setting, light
source and temperature control
• Cleanliness of probes and cuvettes.
• Bacterial contamination of wash water used by the instrument
• Expiry date of the reagent kit **Brand name of Roche Diagnostics, Germany; ***Brand name of Biorad, USA; ▲subject to availability of control serum.
Other Details
Autopure Glucose (Hexokinase) is a ready-to-use, two liquid
reagent system.
Shelf life Unopened reagents are stable till the expiry date indicated
on the label, when stored at 2°- 8°C and protected from light.
Once opened, the reagents are stable up to 60 days at 2° –
8° C, if protected from light and contamination.
Open bottle stability may vary from analyzer to analyzer.
Do not freeze the reagent.
Rina Albert –
Product service is very good
Sonal –
Accurex Glucose Hexokinase Reagent is a ready-to-use,can be used on various automated analyzers.
Sonal –
Accurex Glucose Hexokinase Reagent is a ready-to-use,can be used on various automated analyzers.