AutoPure Calcium Arsenazo III 80*
₹1,838.00
Overview
Buy
AutoPure Calcium Arsenazo III 80
from Accurex (Pack Size: 4 X 20ml). Calcium Arsenazo III 80 Reagent is used for the determination of calcium in human serum and plasma on automated clinical chemistry analyzers.
Calcium Arsenazo III is ready-to-use, single liquid reagent system & the assay is linear upto15 mg/dl (3.75 mmol/l). Arsenazo Ill reacts with calcium ions at neutral pH to form a blue coloured complex.
5 in stock
About
- AutoPure Calcium Arsenazo III is a reagent kit for direct quantitative determination of calcium in human serum and plasma on automated clinical chemistry analyzers.
- AutoPure Calcium Arsenazo III is ready-to-use, single liquid reagent system
- With AutoPure Calcium Arsenazo III, the assay is linear upto 15 mg/dl (3.75 mmol/l).
- Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the calcium results should always be assessed in conjunction with the patient’s medical history, clinical examinations and other findings.
R1 = 250 ul Sample = 5 ul
Principle
Arsenazo Ill reacts with calcium ions at neutral pH to form a blue coloured complex. Neutral pH Ca **+Arsenazo III – Calcium-arsenazo complex Fully automated systems automatically calculate the Calcium concentration of each sample. Results in mmol/l = Results in mg/dlx0.25 With AutoPure Calcium Arsenazo III, the assay is linear upto 15 mg/dl (3.75 mmol/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCl or distilled/deionized water (e.g. 1 + 2). Multiply the result by the appropriate dilution factor(e.g. 3).
Procedure
Serum/Plasma Adults : 8.6- 10.2 mg/dl (2.15-2.55 mmol/l)
Quality Control
Each batch of AutoPure Calcium Arsenazo III is assayed with at least six quality control sera-Precinorm**, Precipath**, Bio Rad I***, Bio Rad II***, Accutrol Normal and Accutrol Abnormal prior to release-, To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together. If the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:
• Proper instrument function – wavelength setting, light source and temperature control.
• Cleanliness of probes and cuvettes.
• Bacterial contamination of wash water used by the instrument.
A comparison of the calcium determination using AutoPure Calcium Arsenazo III and AutoZyme Calcium Arsenazo III reagent gave the following correlation (mg/dl): Linear Regression y=0.949x+0.574 r=0.941 Sy.x=0.33
Other Details
- For calibration it is recommended to use C.f.a.s* or any other suitable calibrator material.
- AutoPure Calcium Arsenazo III is a ready-to-use, single liquid reagent system.
2 reviews for AutoPure Calcium Arsenazo III 80*
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Ramakant Alve –
Sphera FAA with the reagents recommended
Neel –
We have found the Calcium Arsenazo III 80 Reagent to be user-friendly, with clear and concise instructions that simplify reagent preparation and testing procedures.