Principle
Urea is hydrolysed by urease to form ammonia and carbon dioxide.
Quality Control
Each batch of AutoPure Bun/Urea 240
is assayed with at least six quality control sera – Precinom Precipath*, Bio Rad |” Biorad It* Accutrol Normal and Accutrol
Abnormal prior to release
To ensure adequate quality control, it is recommended that the laboratory should Use a normal and abnormal commercial reference control serum. It should Be realized that the use of quality control material checks both the reagent and
instrument functions together.
H the control values fall outside the specified limits, each of the below criteria should be cross checked and corrected:
-Proper instrument function wavelength setting, light sourCe and temperature
-Cleanliness of probes anc cuvettes.
-Bacterial contamination of wash water used by the instrument
-Expiry date of the reagent kt.
-Brand naTIE of Roche Diagnostics, CermaryBrand name a Biorad. USA,subiect to
availability of control serum.
Other Details
AutoPure Urea/BUN is a ready-to-use, two liquid reagent system.
Calibration
There is no significant interference in samples containing upto 60 mg/dl of bilirubin and 700 mg/dl of haemoglobin. Ammonia produced in the cuvette during a GLDH determination interferes with the urea/urea nitrogen assay. The AutoPure Urea/BUN reagent must therefore not be installed on the analyzers together with reagents for the GLDH test. Great care must be taken to prevent ammonia contamination of the specimen and calibrator used in the urea/urea nitrogen assay.
Shelf life Stable till the expiry date indicated on the label, when stored at 20-8°C.
For calibration, it is recommended to use C.f.a.s* or any other suitable calibrator m
Javed Al –
Linear upto 535 mg/dl (88.8 mmol/l)/urea nitrogen 250 mg/dl (41.5 mmol/l).Must buy
Nilesh –
In terms of performance, the Bun/Urea 240 Reagent delivers precise and reproducible measurements of Urea/BUN levels in human serum and plasma.
Mukesh –
The convenience and accuracy offered by this reagent contribute to the overall quality of patient care in our laboratory, making it a valuable asset in our diagnostic arsenal.