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Amylase 60 – Infinite
₹7,454.00
Overview
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Infinite Liquid a-Amylase 60 ml Diagnostic Reagent
from Accurex (LA-2). a-Amylase reagent is used for determination of determination of amylase activity based on kinetic method using Gal G2 – a CNP.
a-Amylase can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzers. a-Amylase Reagent can be determined in just 21/2 minutes at 37°C & is a High Stability Reagent. Accurex Biomedical is the best reagents manufacturing company in India.
5 in stock
About
- Infinite a-Amylase ‘is a reagent set for determination of amylase activity based on kinetic method using Gal G2 – a CNP.
- Infinite a-Amylase is a ready-to-use reagent.
- Infinite a-Amylase can be determined in just 21/2 minutes at 37°C.
- Infinite a-Amylase is linear upto 2000 IU/1.
- Infinite a-Amylase is a High Stability Reagent.
- Infinite a-Amylase can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
Method – Gal-G2-α-CNP - Mix thoroughly and transfer the assay mixture immediately to the thermostated cuvette and start the stopwatch simultaneously. Record the first reading at 60 second and subsequently three more readings with 30 seconds interval at 405 nm.
Principle
Amylase test involves use of a chromogenic substrate Gal G, – a CNP (2-chloro-4-nitrophenyl linked with galactosyl maltoside). The direct action of amylase with this substrate results in the release of more than 90% of 2-chloro-4-nitrophenol, which can be monitored by kinetic assay at 405 nm. The increase in absorbance is directly proportional to the amylase activity in the sample.
Procedure
Reaction type ………………………. Kinetic
Reaction direction …………………..Increasing
Wavelength. ………………………..405 nm
Flow Cell temperature ……………………….. 37° C (+ 0.2°C)
Zero setting with …………………………….. Distilled water
Delay time …………………………… 60 seconds
No. of readings ……………………..4
Interval ……………………………30 seconds
Blank absorbance limit ………………………..< 0.500 Abs
Sample volume ………………………0.02 ml (20)
Linearity …………………………2000 IU/
Quality Control
To ensure adequate quality control, it is recommended that
each laboratory should use a normal and an abnormal
commercial reference control material. It should be realized
that the use of quality control material checks both reagent
and instrument functions together. Factors which might affect
the performance of this test include instrument function,
temperature control, cleanliness of glasswares and accuracy
of pipetting.
Other Details
The kit components should be stored at 2-8°C and is stable til the expiry date indicated on the label. t is recommended to store the reagents at 2-8°C, tightly closed after use.
The working solution (4R1 + 1R2) is to be prepared fresh as per the
requirement.
DO NOT FREEZE THE REAGENTS. Frozen Latex or Diluent could change the functionality of the test.
Contamination of the reagent should be strictly avoided.
1 review for Amylase 60 – Infinite
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Tushar Kokate –
We at Silverline Hospital, Kochi, have been using various products of Accurex Biomedicals since last 3 years..we re very much satisfied with the product quality