Name: Alkaline Phosphatase 60
SKU: 14841
Availability: InStock
Rating: 4.86 (7 reviews)

About
Principle
Procedure
Quality Control
Other Details
Reviews (7)

About

Autozyme Alkaline Phosphatase is a reagent set for determination of Alkaline Phosphatase activity based on kinetic method using p-nitrophenyl phosphate (p-NPP)
Autozyme Alkaline Phosphatase is a single reagent system using one step procedure
Autozyme Alkaline Phosphatase has one step reconstitution. It involves dissolving substrate tablet in diluent
Autozyme Alkaline Phosphatase is a Highly stable reagent
Autozyme Alkaline Phosphatase activity can be determined in just 2.5 minutes

Pack Size : 12 x 5 ml

Principle

The working solution is stablə for 21 days at 2- 8°C.
The working solution should be prepared and stored in the dark (working solution bottle) provided.This is critical because the reagent is light sensitive.
It should therefore be kept away from direct light.
Alkaline Phosphatase cleaves p-nitrophenyl phosphate (p-NPP) into
p-nitrophenol and phosphate. p-nitrophenol is a yellow color compound in alkaline medium and absorbs light at 405 nm. The rate of increase in absorbance at 405 nm. is proportional to Alkaline phosphatase activity in specimen.

Procedure

Reaction type …………………. Kinetic
Reaction direction …………………. Up
Wavelength …………………. 405 nm
Flow cell temperature …………………. 37°C(±0.2°C)
Zero setting with ………………….Working solution
Delay time ………………….60 seconds
No. of readings …………………. 4
Interval …………………. 30 seconds
Blank absorbance limit …………………. < 0.700 Abs.
Sample volume …………………. 0.02 ml (20 µl)
Reagent volume …………………. 1.0 ml
Factor …………………. 2720
Linearity ………………….700 IUl/l

Quality Control

To ensure adequate quality control, it is recommended that each batch should include normal and abnormal commercial reference control serum.
It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and
accuracy of pipetting.

Other Details

The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
The working solutions of Non Prostatic Acid Phosphatase & Total Acid Phosphatases are stable for 7 days at 2-8°C.

7 reviews for Alkaline Phosphatase 60

Rated 5 out of 5
Raushan Sharma – 11/04/2023
We are using accurex reagent from years and getting good result
Rated 5 out of 5
Pinku Patel – 12/05/2023
Superb Products. Always using and most referable products.
Rated 4 out of 5
Vijesh Kanabar – 12/05/2023
Accurex is excellent product. I’m very happy in instrument and reageant using. Quality is very good….
Rated 5 out of 5
Gokul Mhatre – 12/05/2023
Accurex provides good quality products. Accurex is no1 manufacturing company. I am very satisfy with them
Rated 5 out of 5
Pratiksha Kinamay – 12/05/2023
I am using accurex product. Very good and satisfied. I am very happy with accurex biomedicals
Rated 5 out of 5
Abbas Khan – 12/05/2023
Accurex is one of the best company in ivd sector and well known for its quality products
Rated 5 out of 5
Pradeep Pathak – 12/05/2023
Accurex Products are good in quality.

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Alkaline Phosphatase 60

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