About
AccuDx CQ T4 (Thyroxine) Fast Test Kit (Immunofluoreacence Aasay) is intended for in vitro quantitative determination of T4 in serum and plasma. It can be used in the monitoring of hyperthyroid- ism and hypothyroidism, and also used as an aid in the functional diagnosis of thyroidea.
Summary:
The thyroid gland exerts powerful and essential regulatory influences on growth, differentiation, cellar metabolism and general hormonal balance, as well as on the maintenance of metabolic activity and the development of the sketeal and organ system.
T4 is the most commonly measured substance in the assesse- ment of thyroid function. Most of the thyroxine secreted into the bloodstream is bound to a transport protein, thyroxine binding globulin (TBG), and to albumin and pre-albumin. Only less than 1% of thyroxine remains unbound as free T4 in blood. Elevated total thyroxine levels have been associated with hyperthyroidism, a condition with an excess amount of circulating thyroid hormone and decreased total thyroxine levels have been associated with hypothyroidism condition with insufficient levels of thyroxine concentration. Primary malfunction of the thyroid gland or any diseases affecting the thyroid-pituitary-hypothalamus system may result in the abnormal thyroxine concentration in blood. Measurement of thyroxine has been one of the most widely used method for evaluation of an individual’s thyroid status.
Specification
The test uses anti-human T4 monoclonal antibody conjugated with fluorescence latex coated on the junction of nitrocellulose
membrane and sample sad, and T4 antigen coated on the test line. After the sample has been applied to the test strip, the fluorescence latex-labelled anti-human T4 monoconal antibody binds with the T4 in sample and forms marked antigen-antibody
complex. The complex moves to the detection zone by capillary action and then it is captured on the test line by T4
antigen. The fluorescence intensity of the test line increases in proportion to the amount of T4 in sample. Then Insert test card into AccuDxCQ Immunofluorescence Quantitative Analyzer/Automatically inserted by AccuDxCQ Immunofluorescence Quantitative Analyzer (hereafter referred to as AccuDx CQ), the concentrations of T4 in sample will be measured and displayed on the screen. The value will be stored in AccuDx CQ and available for downloading. The result can be easily transmitted to the laboratory or hospital information system.”
Direction for Use
1. Collect specimen according to manual.
2. Test card, sample and reagent should be brought to room temperature prior to testing.
3. Confirm SD card lot No. in accordance with test kit lot No.. Perform “SDCard Calib” calibration when necessary (Details refer to 8.5.2of
Getein1100 User Manual).
4. On the main interface of Getein1100, press “”ENT”” button to enter testing interface.
5. Remove the test card from the sealed pouch immediately before use. Label the test card with patient or control identification.
6. Put the test card on a clean table, horizontally placed.
7. Using sample transfer pipette, deliver 100 μl of sample into one tube of sample diluent, mix gently and thoroughly. Then drop 100 μl (or 3dropsof sample when using disposable pipet) of sample mixture into the sample port on the test card.
8. After 10 minutes, insert the test card into Getein1100 and press “”ENT”” button. The result will be shown on the screen
Other Info
Store the test card at 4~30oC with a valid period of 24 months.
Use the test card within 1 hour once the foil pouch is opened.
Store the blood sample diluent at 0~30oC with a valid period of 24 months.
Store the blood sample diluent at 2~8oC for better results.”
Rajesh Shinde –
Accurex products are trust worthy