About
AccuDx CQ T3 (Triiodothyronine) Fast Test Kit (Immunofluorescenc Assay) is intended for in vitro quantitative determination of T3 in serum and plasma. It can be used in the monitoring of hyperthyroidism and hypothyroidism, and also used as an aid in the functional diagnosis of thyroidea.
Summary:
The thyroid gland exerts powerful and essential regulatory influences on growth, differentiation, cellar metabolism and general hormonal balance, as well as on the maintenance of metabolic activity and the development of the sketeal and organ system. The hormones thyroxine (T4) and triiodothy- ronine (T3) circulate in the blood stream, mostly bound to the plasma protein, throxine binding globulin (TBG). The concentration of T3 is much less than that of T4, but its metabolic potency is much greater.
T3 is produced by the thyroid and secreted in response to TSH.
T3 determination is an important factor in the diagnosis of thyroid disease. Its measurement has uncovered a variant of hyperthyroidism in thyrotoxic patients with elevated T3 levels and normal T4 levels. An increase in T3 without an increase in T4 is frequently a forerunner of recurrent thyrotoxi- cosis in previously treated patients. In other patients, euthyroidism attributable to normal T3, although their T4 values are subnormal.
Specification
Principle:
The test uses anti-human T3 monoclonal antibody conjugated with fluorescence latex coated on the junction of nitrocellulose
membrane and sample sad, and T3 antigen coated on the test line. After the sample has been applied to the test strip, the fluorescence latex-labelled anti-human T3 monoclonal antibody binds with the T3 in sample and forms marked antigen-antibody
complex. The complex moves to the detection zone by capillary action and then it is captured on the test line by T3 antigen. The fluorescence intensity of the test line increases in proportion to the amount of T3 in sample. Then Insert test card into AccuDx CQ Immunofluorescence Quantitative Analyzer the concentrations of T3 in sample will be measured and displayed on the screen. The value will be stored in AccuDxCQ and available for downloading. The result can be easily transmitted to the laboratory or hospital
information system.
Sample diluent composition:
Phosphate buffered saline, proteins, detergent, preservative, stabilizer.
3. A test card consists of: A plastic shell and a reagent strip which is composed of a sample pad, nitrocellulose membrane (the function of membrane and sample pad is coated with fluorescence.
4. Enter testing interface of AccuDx CQ.
5. Remove the test card from the sealed pouch immediatelybefore use. Label the test card with patient or control identification.
6. Put the test card on a clean table, horizontally placed.
7. Using sample transfer pipette, deliver 40 u/ of sample into one tube of sample diluent, mix gently and thoroughly for
1-5 mins. Then drop 100 u/ of the sample mixture into the sample port on the test card.
8. Reaction time: 15 minutes. Insert the test card into AccuDx CQ and start test after reaction time is elapsed. The result will be shown on the screen and printed automatically.
Contents:
1. A kit for AccuDx CQ contains: Package specifications: 25 tests/box, 10 tests/box
1) AccuDx CQ T4 test card in a sealed pouch with desiccant
2) Disposable pipet
3) Sample diluent
4) User manual: 1 piece/box
5) SD card/RFID card: 1 piece/box
2. Sample diluent composition: Phosphate buffered saline, proteins, detergent, preservative, stabilizer.
3. A test card consists of A plastic shell and a reagent strip which is composed of a sample pad, nitrocellulose membrane (the junction of membrane and sample pad is coated with fluorescence latex-labelled anti-human T4 monoclonal antibody, the test lines is coated with T4 antigen, and the control line C is coated with rabbit anti-mouse IgG antibody), absorbent paper and liner.
Note: Do not mix or interchange different batches of kits.
Direction for Use
TEST PROCEDURE
1. Collect specimen according to manual.
2. Test card, sample and reagent should be brought to roomtemperatureprior to testing.
3. Confirm SD card lot No. in accordance with test kit lot No.. Perform“SDCard Calib” calibration when necessary (Details refer to 8.5.2of
Getein1100 User Manual).
4. On the main interface of Getein1100, press “”ENT”” button to enter testinginterface.
5. Remove the test card from the sealed pouch immediately beforeuse. Label the test card with patient or control identification.
6. Put the test card on a clean table, horizontally placed.
7. Using sample transfer pipette, deliver 100 μl of sample into onetubeof
sample diluent, mix gently and thoroughly. Then drop 100 μl (or 3dropsof
sample when using disposable pipet) of sample mixture into thesampleport on the test card.
8. After 10 minutes, insert the test card into Getein1100 and press “”ENT””button. The result will be shown on the screen and
Other Info
STORAGE AND STABILITY:
Store the test card at 4~30oC with a valid period of 24 months.
Use the test card within 1 hour once the foil pouch is opened.
Store the blood sample diluent at 0~30oC with a valid period of 24 months.
Store the blood sample diluent at 2~8oC for better results.
Sanjay Manohar –
Using T3 T4 and TSH in lab