About
1. Infinite LDL-C is a reagent set for direct quantitative determination of LDL-Cholesterol in
2. Human serum or plasma based on the Direct Homogeneous method.
3. Direct measurement by the infinite LDL-C overcomes the limitation of the Fried Ewald formula, thereby allowing accurate determination of LDL-C.
4. Infinite LDL-C is a ready-to-use, two-liquid reagent system stable till expiry.
5. Infinite LDL-C is linear up to 450 mg/dl.
6. There is no significant interference in hyperlipemic and hyperimmunoglobulin samples.
Principle
In the first step, HDL, VLOL & Chylomicrons are eliminated under specific conditions.
In the second step, special reagents break up LDL and make LDL Cholesterol available for measurement.
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference.
SPECIMEN COLLECTION & PRESERVATION
Direction for Use
Reaction type ……… End-Point (2 Step)
Reaction time ………………5 + 5 mins
Wavelength……………. 546 nm.
Flow Cell temperature……………….. 37°C
Zero setting with ……………………… Distilled water
Sample volume ……………. Q.01 ml (10 µt)
Reagent volume 0.750 ml + 0.250 ml
Linearity ………….. .. 450 mg/dl
Other Info
The reagent kit should be stored at 2 – 8°C and is stable till the expiry date indicated on the label. Reconstituted calibrator is stable for 3 days at 2′ – 8°C. Reconstitution stability of the calibrator may be extended upto 30 days by aliquoting and freezing the reconstituted calibrator preparation at less than -20°C.