Principle
Glucose oxidase, oxidizes glucose to gluconic acid in the presence of
atmospheric oxygen.
In presence of peroxidase, hydrogen peroxide oxidatively couples.
with 4-aminoantipyrine and phenol to produce red quinoneimine dye.
The intensity of the red color formed is directly proportional to the
concentration of glucose in the specimen and is measured
photometrically.
Quality Control
Each batch of AutoPure Calcium Arsenazo III is assayed with at least six quality control sera-Precinorm**, Precipath**, Bio Rad I***, Bio Rad II***, Accutrol Normal and Accutrol Abnormal prior to release-, To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together. If the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:
• Proper instrument function – wavelength setting, light source and temperature control.
• Cleanliness of probes and cuvettes.
• Bacterial contamination of wash water used by the instrument.
A comparison of the calcium determination using AutoPure Calcium Arsenazo III and AutoZyme Calcium Arsenazo III reagent gave the following correlation (mg/dl): Linear Regression y=0.949x+0.574 r=0.941 Sy.x=0.33
John K –
I like the product . Good product in cheap price . Quality is good
Deepak –
The reagent’s performance on our analyzers has consistently met our expectations, delivering accurate measurements with minimal variation.