About
- Infinite Microprotein is a reagent set for quantitative determination of protein in human urine & CSF based on Pyrogallol red method.
- Infinite Microprotein is a ready-to-use, single liquid reagent system.
- Infinite Microprotein, is linear upto 300 mg/dL
- Infinite Microprotein assay can be performed in 5 minutes at 37° C.
Method – Pyrogallol Red
Pack Size : 4 * 25 ml
Principle
Pyrogallol red is combined with molybdenum acid at a low pH. When the complex is combined with protein, a blue-purple color is formed. The intensity of the color developed is directly proportional to the protein concentration in the sample and is measured at 600 nm.
Procedure
Reaction type………….………………End-Point
Wavelength………….…….600 nm(578/630 nm)
Flow Cell temperature…………….…………37° C
Zero setting with………….…………….Reagent
Sample volume……….…………..…….……20 µl
Reagent volume…………..………………..1000 µl
Standard Concentration….………….100 mg/dL
Linearity……….…….……………………300 mg/dL
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
– The reagent is ready-to-use.
– The reagent kit should be stored at 2° – 8° C and is stable till the expiry date indicated on the label.
– Keep the Reagent away from direct light sources. Do not freeze the reagent.
– Contamination of the reagent should be strictly avoided.
Hemant Patil –
I m using Accurex Reagents and semi auto with good service