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GOT (AST) 100
₹1,823.00
Overview
Buy
Infinite Liquid GOT (AST) 100 Diagnostic Reagent
(R1 2 X 40ml: R2: 2 X 10ml) from Accurex.
Glutamate Oxaloacetate Transaminase reagent
is used for determination of True Glucose, based on enzymatic method using Glucose, oxidase & Peroxidase.
Accurex Infinite Liquid GOT (AST) can be used on any Spectrophotometer, Discrete semi automated & Automated analyzers. Programme can be designed for any specific analyzer upon request.
50 in stock
About
- Mono-reagent on reconstitution
- Liquid stability : 60 days
- Autozyme GOT is a reagent set for determination of GOT based on UV-Kinetic method recommended by IFCC
- GOT activity can be determined in 2.5 minutes
- Autozyme GOT is linear up to 400 IU/I and has high stability
- Autozyme GOT is a single reagent system, using one step procedure
Pack Size : 20 x 50 ml
Principle
α-ketoglutarate reacts with L-aspartate in presence of GOT (ASTI to form Oxaloacetate and L-glutamate. The increase in oxaloacetate is determined in an indicator reaction catalyzed by malate dehydrogenase. The conversion of NADH to NAD at 340 nm. is proportional to the activity of GOT (AST) in serum/plasma and is determined kinetically as rate of decrease in absorbance.
Procedure
Reaction type ……….. UV – Kinetic
Reaction direction …………….. Down
Wavelength ……………. 340 nm.
Flow Cell temperature…………… 37°C
Zero setting with ……….. Distilled water
Delay time ……….. 60 seconds
No. of readings ……… 4
Interval … 30 seconds
Blank absorbance limit ………………>/ 0.900 Abs.
Sample volume … 0.1 ml (100 ul)
Reagent volume ……1.0 ml
Factor ……1749
Linearity ….. 400 IU/I
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagent kit should be stored at 2- 8°C and is stable till the expiry date indicated on the label.
R, and R, reagents are stable till expiry at 2-8°C.
The working solution (4 R. +1R) is stable for 30 days at 2-8°C..
1 review for GOT (AST) 100
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Rohan Das –
I am using accurex company reagent from last 1 year .this reagent results are very good and I am satisfied for company service