About
Keto-TestGK is a test strip used for semi quantitative estimation of Glucose and Ketone in urine sample.
Keto-lestGK is a disposable, ready to use plastic strip on which are affixed 2 reagent areas for testing : Glucose and Ketone.
When dipped in urine sample the strip colour is read visually, within 40 sec., requiring no additional laboratory equipment for testing.
The Keto-TestGK test strips are packed in an air tight plastic bottle containing a dessicant.
Specification
Glucose : This test is based on a double sequential enzyme reaction. Glucose Oxidase converts urine Glucose to gluconic acid and hydrogen peroxide. Subsequently, Peroxidase converts hydrogen peroxide into water and nascent oxygen. The nascent oxygen liberates iodine from potassium iodide to produce colors ranging from green to brown depending on the urine glucose concentration.
Ketone : The test is based on the principle of Rotheras test. In this reaction, acetoacetic acid in an alkaline medium will react with sodium nitroprusside to produce a purple colour. The term ketones represents three intermediate products of fat metabolism, namely acetone, acetoacetic acid and B-hydroxybutyric acid. The test strip is specific only to acetoacetic acid. It does not measure B-hydroxybutyric acid and is only slightly sensitive to acetone if glycine is present.
Direction for Use
This procedure must be followed exactly to achieve reliable results.
1. Collect fresh, well-mixed, uncentrifuged urine in a clean and dry container.
2. Remove one strip at a time, from the bottle and replace cap immediately and tightly.
3. Dip the reagent areas into urine for 1 to 2 seconds. While removing the strip from the urine run the edge of the strip along the container’s wall and tap to remove excess urine from the strip.
4.Hold the strip close to the color chart provided on the label and match carefully. The glucose results should be read at 30 seconds and the ketone results at 40 seconds. The colour chart should be matched under good light (but not direct sunlight).
Avoid touching the strip directly on the colour chart as this will result in soiling the chart. Interpret the results if the colour obtained falls between two adjacent colour blocks. Colour changes that occur after 40 seconds are of no quantitative diagnostic value.
Other Info
Keto-TestGK is for in vitro diagnostic use. Store the product at room temperature under 35°C. Do not refrigerate. The product should not be used after expiration date.
Mihir Sahu –
Like it v much. Good working. Result is satisfied.