Principle
Infinite Turbilatex RF contain latex particles coated with human
gamma globulin which reacts with RF in the sample resulting in
agglutination, Agglutination causes change in absorbance, measured at 650 nm (600 -650 nm) & is proportional to the concentration of RF in the sample.
Procedure
Reaction type …………. …………….Fixed-Point
Reaction direction ………………..Increasing
Wavelength ……………..540 nm.
Flow Cell temp…………………………..37°C
Zero setting with ……..………. Distilled Water
Delay time ……………………………….5 sec
No. of readings …………………………………2
Interval …. …. ………….120 sec
Sample volume …………………….5 pl
Working solution volume (4R1+1R2) ……………………..1.0 ml a
Linearity.……………150 mg/L
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagent kit should be stored at 2 – 8 C and is stable til the expiry
date indicated on the label. It is recommended to store the reagents at 2°- 8° C, tightly closed after use.
The working solution (9R1 + 1R2) is stable for 30 days at 2°-8°C.
DO NOT FREEZE THE REAGENTS. Frozen Latex or Diluent could
change the functionality of the test.
Contamination of the reagents should be strictly avoided.
Reconstituted calibrator is stable for 1 month at 2-8C or 3 months at – 20°C.
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