About
1. Infinite Turbilatex CRP is a reagent set for quantitative determination of C-Reactive Protein in human serum based on Turbidimetric method.
2. Infinite Turbilatex CRP is a two liquid reagent system using one step procedure.
3. Infinite Turbilatex CRP can be determined in just 2 minutes.
4. Infinite Turbilatex CRP is linear upto 150 mg/L.
5. Infinite Turbilatex CRP can be used on any Spectrophotometer, Discrete semi-automated and automated analyzer.
C-reactive protein is an annular pentameric protein found in blood plasma, whose circulating concentrations rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. high levels of CRP positively correlate with poor outcomes in patients with acute coronary syndrome.
Method – Turbilatex
Principle
Infinite Turbilatex CRP contain latex particles coated with specific anti human CRP which reacts with CRP in the sample resulting in
agglutination. Agglutination causes change in absorbance, measured at 540 nm (530- 550 nm)) & is proportional to the concentration of CRP in the sample.
Procedure
Reaction type ………………………..Fixed-Point
Reaction direction ………………..Increasing
Wavelength ……………..540 nm.
Flow Cell temp…………………………….37°C
Zero setting with ……… Distilled Water
Delay time ………………………………..5 sec
No. of readings ………………………………….2
Interval …………………120 sec
Sample volume …………………….5 pl
Working solution volume (4R1+1R2) ……………………..1.0 ml a
Linearity……………..150 mg/L
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagent kit should be stored at 2 – 8°C and is stable till the expiry date indicated on the label. It is recommended to store the reagents at 2-8°C, tightly closed after use.
The working solution (9R1 + 1R2) is stable for 30 days at 2- 8°C.
DO NOT FREEZE THE REAGENTS. Frozen Latex or Diluent could
change the functionality of the test.
Contamination of the reagents should be strictly avoided.
Reconstituted calibrator is stable for 1 month at 2- 8°Cor 3 months at -20°C.
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