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Triglyceride 50
₹1,823.00
Overview
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Infinite Liquid Triglyceride 50 Diagnostic Reagent
(Pack Size: 2 X 25 ml) from Accurex. Liquid Triglycerides reagent kit is used for determination of triglycerides, based on enzymatic methods using Lipoprotein lipase, Glycerol kinase, Glycerol phosphate oxidase and Peroxidase. Triglyceride Reagent is a ready to use reagent & can be determined in just 10 minutes at 37°C or 20 minutes at Room Temperature (25°C – 30°C). The Triglyceride kit should be stored at 2- 8°C and is stable till the expiry date indicated on the label.
Triglycerides normal range
is Less than 150 milligrams per deciliter (mg/dL) or less than 1.7 millimoles per liter (mmol/L).
50 in stock
About
- Infinite Liquid Triglycerides 125 is a reagent set for determination of triglycerides, based on enzymatic methods using Lipoprotein lipase, Glycerol kinase, Glycerol phosphate oxidase and Peroxidase.
- Infinite Liquid Triglycerides 125 is a ready to use reagent.
- Triglycerides 125 can be determined in just 10 minutes at 37°C or 20 minutes at R.T. (25°C – 30°C).
- Infinite Liquid Triglycerides 125 is linear upto 800 mg%.
- Infinite Liquid Triglycerides 125 can be used on any Colorimeter, Spectrophotometer, Discrete semi automated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
- The influence of lipids, haemolysis and bilirubin (upto 8 mg%) is negligible.
Method – GPO-POD
Principle
Glycerol released from hydrolysis of triglycerides by lipoprotein lipase is Converted by glycerol kinase into glycerol- 3- phosphate which is oxidized by glycerol phosphate oxidase to dihydroxyacetone phosphate and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidizes phenolic chromogen to a red coloured compound.
Procedure
Reaction type ………… ………. End-Point
Reaction time …. 10 mins. at 37°C/20 mins. at R.T. (25-30°C)
Wavelength ……….. ………… 510 nm. (500 – 530 nm.)
Zero setting with ……….. ………… Reagent Blank
Blank absorbance limit … ……… < 0.300 Abs.
Sample volume …………………………… 0.01 ml (10 jul)
Reagent volume ……….. …………. 1.0 ml
Standard concentration .. …………… 200 mg%
Linearity ……………. ….. 800 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The kit should be stored at 2- 8°C and is stable till the expiry date indicated on the label.
The reagent and standard are ready-to-use and are stable till expiry, when stored at 2-8°C. DO NOT FREEZE THE REAGENT.
The reagent should be stored only in the amber bottle provided to protect it from direct light. Before use swirl in the reagent gently. DO NOT SHAKE VIGOROUSLY.
Over time, the reagent may develop a light pink color. This is expected and does not affect the reagent performance. Discard the reagent if the absorbance of the same exceeds 0.300O.D. against distilled water at 510 nm.
Contamination of the reagent should be strictly avoided. Should the
reagents develop turbidity and discard the reagent.
5 reviews for Triglyceride 50
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