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T HDL – Cholesterol (Direct) 320 – AutoPure
₹38,770.00
Overview
Buy AutoPure T HDL – Cholesterol (Direct) 320 (Pack Size: 2 X 60ml) from Accurex.
AutoPure T HDL-Cholesterol assay
is a homogeneous method for directly measuring HDL-C levels in serum/plasma without the need for any off line pretreatment or centrifugation step.
T HDL – Cholesterol reagent kit is used for direct quantitative determination of high-density lipoprotein cholesterol (HDL-C) in human serum and plasma on automated clinical. AutoPure T HDL-C is a ready-to-use 2 liquid reagent system & with AutoPure T HDL-C, the assay is linear upto 150 mg/dl (3.89 mmol/l).
50 in stock
About
- AutoPure T HDL-C is a reagent kit for direct quantitative determination of high-density lipoprotein cholesterol (HDL-C) in human serum and plasma on automated clinical chemistry analyzers.
- AutoPure T HDL-C is a ready-to-use, two liquid reagent system.
- With AutoPure T HDL-C, the assay is linear upto 150 mg/dl (3.89 mmol/l).
Principle
AutoPure T HDL-Cholesterol assay is a homogeneous method for directly measuring HDL-C levels in serum or plasma without the need for any off-line pretreatment or centrifugation steps. In the first reaction, non HDL unesterified cholesterol is subject to an enzyme reaction and the peroxide generated is consumed by a peroxidase reaction with DSBmT to yield a colorless product. The second reagent consists of a detergent capable of solubilizing HDL specifically. Cholesterol esterase and a chromogenic coupler reacts with this solubilized HDL-C to develop colour.
The intensity of color formed is directly proportional to the concentration of HDL-C.
Procedure
For calibration, it is recommended to use T HDL-C Calibrator from Accurex. Other commercially available HDL-C calibrators have not been tested with this assay and cannot be supported by Accurex Biomedical Pvt. Ltd. Refer to the T HDL-C Calibrator kit package insert for a description of assignment procedures and instructions. The value of the T HDL-C Calibrator was assigned by procedures traceable to National Reference System for Cholesterol (NRS/CHOL). Calibration material have concentrations around the medical decision level.
Quality Control
Each batch of AutoPure T HDL-C is assayed with multiple quality control sera prior to release. To ensure adequate quality control, it is recommended that the laboratory should use a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both reagent and instrument functions together. The value of these controls should fall within the specified limits. If control values fall outside specified limits, each of the below criteria should be cross-checked and corrected :
• Proper instrument function – wavelength setting, light source and
temperature control
• Cleanliness of probes & cuvettes
• Bacterial contamination of wash water used by the instrument
• Expiry date of the reagent kit
Other Details
AutoPure T HDL-C can be used on various automated analyzers.
5 reviews for T HDL – Cholesterol (Direct) 320 – AutoPure
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